Horticultural Science, 2004 (vol. 31), issue 4
Production and utilization of doubled haploids in Brassica oleracea vegetables
M. Klíma, M. Vyvadilová, V. Kučera
Hort. Sci. (Prague), 2004, 31(4):119-123 | DOI: 10.17221/3804-HORTSCI 
A possibility to increase the efficiency of plant regeneration from microspore-derived embryos of selected botanical varieties of Brassica oleracea was investigated from 2001 to 2004. More than 400 regenerants of R1 generation were derived in kohlrabi, cabbage and cauliflower by means of different modifications of microspore culture technique. Distinct genotype differences in embryogenic responsibility and regenerative ability of microspore embryos to whole plants were detected. The highest frequency of embryogenesis and subsequent regeneration of plants were achieved in cauliflower cultivar Siria F1, kohlrabi line P7 and some experimental...
The effect of growth regulators on the rooting of shoots of the peach rootstock Ishtara in in vitro conditions
A.W. Alsalihy, B. Křižan, M. Klemš, H. Fišerová, J. Hradilík
Hort. Sci. (Prague), 2004, 31(4):124-131 | DOI: 10.17221/3805-HORTSCI
The effect of indolyl-3-butyric acid (IBA) and paclobutrazol (PP 333) on the rooting of shoots of the Ishtara peach rootstock was examined in in vitro conditions in light. In the first stage of the experiments the effect of the IBA concentration (0, 0.75, 1.5 mg/l) was studied and in the second stage the effect of the interaction of IBA (0.75 mg/l) and PP 333 (0.06 and 0.12 mg/l) in the MS medium, which is optimal for the rooting of the peach rootstock Ishtara. During shoot cultivation we evaluated root formation (average length and number). During rhizogenesis the production of ethylene, ethane and CO2 by the shoots and the level...
Demonstration of lactase activity in culture medium of melon cells
J. Stano, K. Mičieta, E. Tokhtaeva, M. Valšíková, M. Koreňová, V. Blanáriková
Hort. Sci. (Prague), 2004, 31(4):132-135 | DOI: 10.17221/3806-HORTSCI
Lactase activity was detected in a culture medium of the cell suspension culture of watermelon (Citrullus vulgaris L.). A simple, rapid and reproducible procedure for identification of extracellular lactase is described using callus cultures of seedlings from the tested plant, hairy roots of 2.5 days old seedlings of watermelon germinating on agar plates as well as cell suspension cultures derived from callus cultures. For the determination of intracellular activities of lactase, 6-bromo-2-naphthyl-β-D-galactopyranoside and p-nitrophenyl-β-D-galactopyranoside were used as synthetic substrates. The extracellular lactase activity...
The RAPD analysis of several cultivars of grapevine (Vitis viniferaL.) and their clones
H. Vlastníková, K. Moravcová, M. Pidra
Hort. Sci. (Prague), 2004, 31(4):136-139 | DOI: 10.17221/3807-HORTSCI
Nine identification RAPD markers (Moravcová et al. 2003) were used to distinguish 24 clones and grapevine cultivars. No polymorphism was detected among all the tested clones of Chardonnay, Pinot gris and Zweigeltrebe from Polešovice. Pinot noir, Pinot gris, Pinot blanc and Pinot Meunier were indistinguishable within clones, they also showed the identical RAPD profile within cultivars (except discussed sample No. 26). On the other hand, Auxerrois as a relative to cultivars of Pinot group showed unique patterns and may be classified as a different cultivar. Some irregularities within the cultivars of Pinot family from Oblekovice were also found, several...
Protoplast cultures and protoplast fusion focused on Brassicaceae: A review
B. Navrátilová
Hort. Sci. (Prague), 2004, 31(4):140-157 | DOI: 10.17221/3809-HORTSCI
The subjects of this article are protoplast isolations and protoplast fusions, in particular their history, a review of factors influencing the protoplasts isolation and fusion, selection of hybrid plants and utilization of somatic hybrids in plant breed-ing. Somatic hybridization through protoplast fusion can overcome sexual incompatibility among plant species or genera; transfer genes of resistance to diseases (viral, bacterial, fungal), pests, herbicides and others stress factors; obtain cybrid plants; transfer cytoplasmic male sterility or incease content of secondary metabolites in hybrid plants. The article is focussed mainly on the family Brassicaceae...