Hort. Sci. (Prague), 2004, 31(4):124-131 | DOI: 10.17221/3805-HORTSCI

The effect of growth regulators on the rooting of shoots of the peach rootstock Ishtara in in vitro conditions

A.W. Alsalihy, B. Křižan, M. Klemš, H. Fišerová, J. Hradilík
Department of Botany and Plant Physiology, Mendel University of Agriculture and Forestry, Brno, Czech Republic

The effect of indolyl-3-butyric acid (IBA) and paclobutrazol (PP 333) on the rooting of shoots of the Ishtara peach rootstock was examined in in vitro conditions in light. In the first stage of the experiments the effect of the IBA concentration (0, 0.75, 1.5 mg/l) was studied and in the second stage the effect of the interaction of IBA (0.75 mg/l) and PP 333 (0.06 and 0.12 mg/l) in the MS medium, which is optimal for the rooting of the peach rootstock Ishtara. During shoot cultivation we evaluated root formation (average length and number). During rhizogenesis the production of ethylene, ethane and CO2 by the shoots and the level of abscisic acid (ABA) in the shoot base was monitored in in vitro conditions. Shoots cultivated on the MS medium with 1.5 mg/l of IBA produced the highest number of roots and the longest roots that produced the lowest level of ethylene and CO2 and the level of ABA was the lowest in the bases.

Keywords: IBA; PP 333; ethylene; ABA; Prunus persica L.; rooting in vitro

Published: December 31, 2004  Show citation

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Alsalihy AW, Křižan B, Klemš M, Fišerová H, Hradilík J. The effect of growth regulators on the rooting of shoots of the peach rootstock Ishtara in in vitro conditions. Hort. Sci. (Prague). 2004;31(4):124-131. doi: 10.17221/3805-HORTSCI.
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    References

    1. ALSALIHY A.W., KŘIŽAN B., FIŠEROVÁ H., HRADILÍK J., 2003a. The problems of propagation of peach rootstocks (Prunus persica L.) in in vitro conditions. Brno, Mendel University of Agriculture and Forestry, Faculty of Agronomy - MendelNet 03.
    2. ALSALIHY A.W., KŘIŽAN B., FIŠEROVÁ H., HRADILÍK J., 2003b. The effect of four different media on in vitro multiplication of peach rootstocks. In: GAJDOŠOVÁ A., LIBIAKOVÁ G. (eds.), Proc. 5th Symposium Recent Advances in Plant Biotechnology, Stará Lesná, Slovak Republic: 28.
    3. ALVES A.A.C., SETTER T.L., 2000. Response of cassava to water deficit: Leaf area growth and abscisic acid. Crop Sci., 40: 131-137. Go to original source...
    4. BALLA I., VERTESY J., VEGVARI G., SZUCS E., KALLAY T., VOROS I., BORO B., 2003. Nutrition of micropropagated fruit trees in vitro and ex vitro. Int. J. Hort. Sci., 9: 43-46. Go to original source...
    5. BETTIN A., FAUPEL H., 1990. Introduction of CO2 during azalea propagation, better rooting through feeding with CO2. Gartnerborse-und-Gartenwelt, 90: 256-258.
    6. BLAKE J.I., LINDERMAN R., LAVENDER D., 1988. Seedling vigour of Douglas fir and western hemlock in relation to ethylene exposure levels and ethane production during cold storage. Proc. 10th North American Forest Biology Workshop, Physiology and Genetics of Reforestation, Vancouver, British Columbia, July 10-22: 235-242.
    7. CELIKEL F.G., VANDOORN W.G., 1995. Solute leakage, lipid peroxidation and protein degradation during the senescence of Iris tepals. Physiol. Plant., 94: 515-521. Go to original source...
    8. CLARK D.G., GUBRIUM E.K., BARRETT J.E., NELL T.A., KLEE H.J., 1999. Root formation in ethylene-insensitive plants. Plant Physiol., 121: 53-59. Go to original source... Go to PubMed...
    9. DAVIES W.J., TARDIEU F., TREJO C.L., 1994. How do chemical signals work in plants that grow in a drying soil? Plant Physiol., 104: 309-314. Go to original source... Go to PubMed...
    10. DAVIS T.D., HAISSIG B.E., SANKHLA N., 1988. Adventitious root formation in cuttings. Portland, Diocorides Press. In: PROCHÁZKA S., ŠEBÁNEK J. (eds.), Regulátory rostlinného růstu. Praha, Academia: 194-204.
    11. DIMASI-THERIOU K., ECONOMOU A.S., SFAKIOTAKIS E.M., 1993. Promotion of petunia (Petunia hybrida L.) regeneration in vitro by ethylene. Plant Cell Tiss. Org. Cult., 32: 219-225. Go to original source...
    12. DOSTÁL R., 1912. Rozpravy Čes. Akad. No. II, 21: 1-38.
    13. DRIVER J.A., KUNIYUKI A.H., 1984. In vitro propagation of walnut rootstock. Hort. Sci., 19: 507-509. Go to original source...
    14. DRUAT P., 1980. Plantlet regeneration from callus of different Prunus species. Sci. Hort., 12: 339-342. Go to original source...
    15. ENGLAND D.J.F., 1978. PP 333 - A new grass retardant. In: RORISON I.H., HUNT R. (ed.), Amenity Grassland: An Ecological Perspective. Chichester, Wilei: 234.
    16. FARI M., KERTÉZ T., FRANKLIN DE MĚLO N., ANDRÁSFALVY A., 1996. Novel tendencies of automation and rationalization of in vitro explant manipulation in micropropagation industry. Hort. Sci., 28: 12-17.
    17. FIGUERIA A., WHIPKEY A., JANICK J., 1991. Increased CO2 and light in vitro shoot growth and development of Theobroma cacao. J. Amer. Soc. Hort. Sci., 116: 585-589. Go to original source...
    18. FIŠEROVÁ H., HRADILÍK J., 1994. Produkce etylénu a etanu při tvorbě adventivních kořenů na stonkových segmentech révy vinné. Rostl. Výr., 40: 755-762.
    19. FIŠEROVÁ H., KULA E., KLEMŠ M., REINÖHL V., 2001. Phytohormones as indicators of the degree of damage in birch (Betula pendula). Biológia (Bratislava), 56: 405-409.
    20. FRANC P., 1998. The effect of auxin and their cofactors on root formation in peach rootstocks in vitro. Hort. Sci. (Prague), 25: 41-46.
    21. GASPAR T., 1990. Plant micropropagation: Progressively out of the empiricism. 4th Forum for Applied Biotechnology, 27. Ghent. Mededeingen van de Faculteit Landbouwwetenschappen, Rijksuniversiteit Gent, 55: 1385-1392.
    22. GRANT W.J.R., FAN H.M., DOWTON W.J.S., LOVEYS B.R., 1992. Effect of CO2 enrichment on the physiology and propagation of two Australian ornamental plants, Chamelaucium uncinatum (Schauer) × Chamelaucium floriferum (MS) and Correa schlechtendalii (Behr). Sci. Hort., 52: 337-342. Go to original source...
    23. HADID A.F.A., 1992. The relationship between ethylene and auxin on adventitious root initiation in cuttings in cucumber. International symposium on transplant production systems. Biological, engineering and socioeconomic aspects, Yokohama, Japan. Acta Hort., 319: 447-452. Go to original source...
    24. HOAD G.V., 1975. Effect of osmotic stress on abscisic acid levels in xylem sap of sunflower (Helianthus annuus L.). Planta, 124: 25-29. Go to original source... Go to PubMed...
    25. HRADILÍK J., FIŠEROVÁ H., 1986. Interakce mezi giberelinem (GA3) a paclobutrazolem (PP 333) u salátu, hrachu a lnu. Acta Univ. Agric. (Brno), Fac. Agron. XXXV, 4: 5-11.
    26. HRADILÍK J., FIŠEROVÁ H., 1987. Vliv kyseliny giberelové (GA3) a paclobutrazolu (PP 333) na morfologii bramboříku. Acta Univ. Agric. (Brno), Fac. Agron. XXXV, 4: 23-26.
    27. JONES O.P., HOOPGOOD E., 1979. The successful propagation of in vitro rootstocks of Prunus: the plum rootstock pixy (P. institia) and the cherry rootstock F12/1 (P. avium). J. Hort. Sci., 54: 63-66. Go to original source...
    28. KARHU S.T., ULVINEN S.K., 1995. The effect of different carbohydrates on the rooting of micropropagation apple shoots and their adaptation after transplantation. Bull. Rech. Agron. Gembloux, 30: 87-101.
    29. KRÁLÍK J., ŠEBÁNEK J., 1989. Vliv paclobutrazolu na rhizogenezi řízků některých dřevin. Acta Univ. Agric., Brno, A, 37: 51-54.
    30. LA MOTTE C., LI X., JACOBS W., EPSTEIN E., 2002. Quantitative relationship between indole-3-acetic acid and abscisic acid during leaf growth in Coleus blumei. Plant Growth Regulation, 36: 12-25. Go to original source...
    31. MARINO G., VENTURA M., 1997. The influence of ethylene on in vitro rooting of GF 677 (Prunus persica × Prunus amygdalus) hybrid peach rootstock. In vitro Cellularand Devel. Biol. Plant, 33: 26-29. Go to original source...
    32. MONCOUSIN C., FAVRE J.M., GASPAR T., 1989. Early changes in auxin and ethylene production in vine cuttings before adventitious rooting. Plant Cell, Tiss. Org. Cult., 19: 235-242. Go to original source...
    33. MURASHIGE T., SKOOG F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant., 15: 473-497. Go to original source...
    34. NÉMETH G., 1981. Adventitious root induction by substituted 2-chloro-3-phenylpropionnitrilies in apple rootstocks cultured in vitro. Z. Pflanzenphysiol., 95: 389-396. Go to original source...
    35. OKADA K., 2003. Shot growth and rooting of cherry tree plantlets after the addition of carbon dioxide generated from bath conditioners. J. Soc. High Technol. Agric., 15: 39-42. Go to original source...
    36. ORLIKOWSKA T., 1992. Influence of arginine on in vitro rooting of dwarf apple rootstocks. Plant Cell Tiss. Org. Cult., 31: 9-14. Go to original source...
    37. PSOTA V., FIŠEROVÁ H., LÁNIČKOVÁ B., 1997. Changes in IAA and ethylene levels in Salix babylonica during rhizogenesis. Biológia (Bratislava), 52: 561-566.
    38. QUARRIE S.A., WHITFORD P.N., APPLEFORD N.E.J., WANG T.L., COOK S.K., HENSON L.E., LOVEYS B.R., 1988. A monoclonal antibody to (S)-abscisic acid: its characterisation and use in a radioimunoassay for measuring abscisic acid in crude extracts of cereal and lupin leaves. Planta, 183: 330-339. Go to original source... Go to PubMed...
    39. RADIN J.W., LOOMIS R.S., 1969. Ethylene and carbon dioxide in the growth and development of cultured radis roots. Plant Physiol., 44: 1584-1589. Go to original source... Go to PubMed...
    40. RALLO L., RIO C., 1990. Effect of a CO2-enriched environment on the rooting ability and carbohydrate level of olive cuttings. Advances Hort. Sci., 4: 129-130.
    41. RIOV J., YANG S.F., 1989. Ethylene and auxin-ethylene interaction in adventitious root-formation in mung bean cuttings. J. Plant Growth Regulation, 8: 131-141. Go to original source...
    42. ROUT G.R., DAS P., 1999. Effect of AgNO3 on high frequency plant regeneration of Simarouba glauca Linn. Angewandte Botanik, 73: 15-19.
    43. SPOLLEN W.G., LENOBLE M.E., SAMUELS T.D., BERNSTEIN N., SHARP R.E., 2000. Abscisic acid accumulation maintains maize primary root elongation at low water potentials by restricting ethylene production. Plant Physiol., 122: 967-976. Go to original source... Go to PubMed...
    44. ŠEBÁNEK J., KLÍČOVÁ Š., KRÁLÍK J., PSOTA V., VITKOVÁ H., KUDOVÁ D., REINÖHL V., 1991. The effect of paclobutrazol on the level of endogenous IAA in relation to the rooting of cuttings and abscission of petioles. Biochem. Physiol. Pflanz., 187: 89-94. Go to original source...
    45. TARI I., NAGY M., 1996. Abscisic acid and Ethrel abolish the inhibition of adventitious root formation of paclobutrazol-treated bean primary leaf cuttings. Biologia Plant., 38: 369-375. Go to original source...
    46. THIMANN K.V., WENT F.W., 1934. On the chemical nature of the root-forming hormone. In: Proc. Con. Ned. Acad. Wetensch, Amsterdam, 37: 456-459.
    47. VACHŮN Z., 1996. Ovocnictví - Podnože ovocných dřevin. Brno, MZLU: 67.
    48. ZIMMERMANN P.W., HITCHCOCK A.E., 1933. Initiation and stimulation of adventitious roots caused by unsaturated hydrocarbon gases. Contrib. Boyce Thomson Inst., 5: 351-369.

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