Hort. Sci. (Prague), 2004, 31(4):136-139 | DOI: 10.17221/3807-HORTSCI

The RAPD analysis of several cultivars of grapevine (Vitis viniferaL.) and their clones

H. Vlastníková, K. Moravcová, M. Pidra
Faculty of Horticulture, Mendel University of Agriculture and Forestry, Brno, Lednice, Czech Republic

Nine identification RAPD markers (Moravcová et al. 2003) were used to distinguish 24 clones and grapevine cultivars. No polymorphism was detected among all the tested clones of Chardonnay, Pinot gris and Zweigeltrebe from Polešovice. Pinot noir, Pinot gris, Pinot blanc and Pinot Meunier were indistinguishable within clones, they also showed the identical RAPD profile within cultivars (except discussed sample No. 26). On the other hand, Auxerrois as a relative to cultivars of Pinot group showed unique patterns and may be classified as a different cultivar. Some irregularities within the cultivars of Pinot family from Oblekovice were also found, several of them gave different results from those expected: Pinot blanc sample 26 has the RAPD profile typical of Chardonnay. A new abnormal RAPD pattern as a marker of typical Chardonnay and Pinot profiles was observed in two cases. While RAPD banding patterns could not distinguish between the known clones, they were useful for distinguishing between phenotypically similar cultivars and for assessing the origins of cultivars thought to have originated as sports.

Keywords: grapevine; clones; fingerprinting; RAPD

Published: December 31, 2004  Show citation

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Vlastníková H, Moravcová K, Pidra M. The RAPD analysis of several cultivars of grapevine (Vitis viniferaL.) and their clones. Hort. Sci. (Prague). 2004;31(4):136-139. doi: 10.17221/3807-HORTSCI.
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    References

    1. BOWERS J.E., BANDMAN X., MEREDITH C.P., 1993. DNA fingerprint characterization of some wine grape cultivars. Amer. J. Enol. Vitic., 44: 266-274. Go to original source...
    2. CASTIGLIONE S., WANG G., DAMIANI G., BANDI C., BISOFFI S., SALA F., 1993. RAPD fingerprints for identification and for taxonomic studies of elite poplar (Populus spp.) clones. Theor. Appl. Genet., 87: 54-59. Go to original source... Go to PubMed...
    3. GRANDO M.S., FRISINGHELLI C., 1998. Grape microsatellite markers: Sizing of DNA alleles and genotype analysis of some grapevine cultivars. Vitis, 37: 79-82.
    4. GRANDO M.S., 2002. Grape Microsatellite collection (online database) San Michele all'Adige - Italy: Istituto Agrario San Michele all'Adige (IASMA) <http://217.222.71.209/genetica/query.html >
    5. HU J., QUIROS C.F., 1991. Identification of broccoli and cauliflower cultivars with RAPD markers. Plant Cell Rep., 10: 505-511. Go to original source... Go to PubMed...
    6. MAILER R.J., SCARTH R., FRISTENSKY B., 1994. Discrimination among cultivars of rapeseed Brassica napus L. using DNA polymorphism amplified from arbitrary primers. Theor. Appl. Genet., 87: 697-704. Go to original source... Go to PubMed...
    7. MORAVCOVÁ K., BARÁNEK M., PIDRA M., 2004. The use of RAPD markers for differentiation of grapevine cultivars registered in the Czech Republic. Hort. Sci., 31: 102-107. Go to original source...
    8. MORAVCOVÁ K., BARÁNEK M., SOTOLÁŘ R., PIDRA M., 2003. Identification of grapevine varieties by moleculargenetic method RAPD. Science of the Youth, 6.-7. 9. 2003, Coll. of Abstracts, B48: 276.
    9. MORENO S., GOGORCENA Y., ORTIZ J.M., 1995. The use of RAPD markers for identification of cultivated grapevine (Vitis vinifera L.). Sci. Hort., 62: 237-243. Go to original source...
    10. REGNER F., SEFC K.M., GLÖSSL J., STEINKELLNER H., 2000a. Parentage analysis and pedigree reconstruction of vine cultivars using microsatellite markers. Acta Hort., VII International Symposium on Grapevine Genetics and Breeding: 177-183. Go to original source...
    11. REGNER F., WIEDECK E., STADLBAUER A., 2000b. Differentiation and identification of White Riesling clones by genetic markers. Vitis, 39: 103-107.
    12. REGNER F., STADBLAUER A., EISENHELD C., 2001. Molecular markers for genotyping grapevine and for identifying clones of traditional varieties. Acta Hort., 546: 331-341. SEFC K.M., REGNER F., GLÖSSL J., STEINKELLNER H., 1998. Genotyping of grapevine and rootstock cultivars using microsatellite markers. Vitis, 37: 15-20. Go to original source...
    13. WELSH J., McCLELLAND M., 1990. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res., 19: 303-306. Go to original source... Go to PubMed...
    14. WILLIAMS J.G.K., KUBELIK A.R., LIVAK K.J., RAFALSKI J.A., TINGEY S.V., 1990. DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res., 18: 6531-6535. Go to original source... Go to PubMed...
    15. YE G.N., SOYLEMEZOGLU G., WEEDEN N.F., LAMBOY W.F., POOL R.M., REISCH B.I., 1998. Analysis of the relationship between grapevine cultivars, sports and clones via DNA fingerprinting. Vitis, 37: 33-39.
    16. YU L.X., NGUYEN T.H., 1994. Genetic variation detected with RAPD markers among upland and lowland rice cultivars (Oryza sativa L.). Theoret. Appl. Genet., 87: 668-672. Go to original source... Go to PubMed...

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