Hort. Sci. (Prague), 2025, 52(2):131-143 | DOI: 10.17221/62/2024-HORTSCI

Evaluation of different rooting stimulators and substrates to produce guava clone plants through softwood cuttingsOriginal Paper

Muhammad Tahir Akram ORCID...1, Rashad Qadri2, Mehwish Liaquat1, Naveed ur Rehman1, Tanveer Hussain1, Irfan Ali1, Sarvet Jehan3, Muhammad Azam2, Muhammad Mumtaz Khan4, Muhammad Ahsan Khatana5, Arslan Achakzai6, Israr Ali1
1 Department of Horticulture, PMAS-Arid Agriculture University, Rawalpindi 46300, Pakistan.
2 Institute of Horticultural Sciences, University of Agriculture, Faisalabad, Pakistan.
3 Institute of Soil and Environmental Sciences, PMAS-Arid Agriculture University, Rawalpindi 46300, Pakistan.
4 Department of Plant Sciences, College of Agriculture and Marine Science, Sultan Qaboos University, P.O. Box 34, Al-khoud 123, Oman
5 Plant Genetic Resources Institute, National Agricultural Research Centre, Pakistan.
6 Directorate of Agriculture Research Fruit, Agriculture Research Institute (ARI), Quetta, Pakistan

The clonal propagation of guava (Psidium guajava L.) is essential to avoid segregation, and its multiplication through cuttings is the easiest and cheapest way to raise a nursery, as other propagation techniques are laborious and need expertise. The current study was planned to evaluate the best rooting stimulators and rooting substrates for guava nursery propagation. In this experiment, the guava-prepared cuttings were treated with rooting stimulators, indole butyric acid (IBA) and paclobutrazol (PB) with concentrations (0, 200, 400, and 600 ppm) and were planted in independent rooting substrates (sand, silt and topsoil) under polytunnels. The results revealed that the highest number of roots, root length and root weight were achieved in cuttings planted in the sand with IBA 400 ppm concentration. In contrast, the highest survival percentage (50%) was obtained in silt substrate with a similar concentration of IBA. However, sand’s lowest and highest concentrations of both rooting hormones produced zero plants. In shooting capacity, maximum leaves (13.6) and sprouts (3.76) were obtained using IBA 400 ppm in silt substrate. The highest sprouting length (6.80 cm) and cutting height (26.2 cm) were attained with sand using PB 200 ppm. In the overall comparison, rooting stimulator IBA 400 ppm and rooting substrate silt performed better than other rooting stimulators and rooting substrates.

Keywords: clonal propagation; indole butyric acid; paclobutrazol; polytunnels; rooting

Received: March 31, 2024; Revised: October 10, 2024; Accepted: October 25, 2024; Prepublished online: May 23, 2025; Published: June 26, 2025  Show citation

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Akram MT, Qadri R, Liaquat M, Rehman NU, Hussain T, Ali I, et al.. Evaluation of different rooting stimulators and substrates to produce guava clone plants through softwood cuttings. Hort. Sci. (Prague). 2025;52(2):131-143. doi: 10.17221/62/2024-HORTSCI.
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