Horticultural Science, 2021 (vol. 48), issue 2
Pathogenic and biological characterisation of T-DNA insertional mutants of a Colletotrichum gloeosporioides casual organism of apple anthracnoseOriginal Paper
Muhammad Azeem, Zongshan Zhou, Junxiang Zhang, Muhammad Ibrahim Khaskheli, Ji Zhi Rui, Allah Jurio Khaskheli, Safdar Ali
Hort. Sci. (Prague), 2021, 48(2):51-62 | DOI: 10.17221/82/2020-HORTSCI 
Anthracnose leaf spot caused by Colletotrichum gloeosporioides is an important disease of apples, resulting in serious damage to the fruit production. In this paper, the pathogenic and physiological characters of sixteen isolates and the wild isolate "Stj16" were studied. In the current study, we generated C. gloeosporioides strains expressing green fluorescence by introducing a GFP gene via an Agrobacterium tumefaciens-mediated transformation (ATMT). To confirm the subcellular localisation of the A2799 gene, an A2799gfp fusion expression mutant was constructed. After observation of the fusion expression, the A2799gfp fusion expression protein was...
Establishment of an efficient micropropagation system in enhancing rooting efficiency via stem cuttings of apple rootstock M9T337Original Paper
Jiangli Shi, Zhidan Dong, Chunhui Song, Beiyang Xie, Xianbo Zheng, Shangwei Song, Jian Jiao, Miaomiao Wang, Tuanhui Bai
Hort. Sci. (Prague), 2021, 48(2):63-72 | DOI: 10.17221/106/2020-HORTSCI
Rootstocks play a vital role in regulating the environmental adaptability and controlling the growth and development of apple trees. M9T337, an excellent apple rootstock widely used in commercial orchards, could confer dwarf tree architectures, early fruiting and suitability for high-density planting. However, the rooting ability of M9T3337 is low when it is vegetatively propagated, and researchers have not yet established an efficient micropropagation system. The present study systematically evaluated the multiplication in adventitious shoots and the in vitro formation of adventitious roots to determine the effects of the culture media and plant growth...
The effect of the short pruning on the yield and quality of the fruits at the peach treeOriginal Paper
Nikola Saraginovski, Marjan Kiprijanovski
Hort. Sci. (Prague), 2021, 48(2):73-79 | DOI: 10.17221/158/2019-HORTSCI
The aim of our research was to determine the effect of short pruning on the productive and vegetative characteristics of peach trees. The research was conducted on two cultivars, 'Redhaven' and 'Fayette'. Three short pruning treatments were applied, with heading of the bearing branches at 20, 30, and 40 cm in length and a control, without heading. The following characteristics were evaluated: the growth of the TCSA, the number of thinned fruitlets, the number of fruit and yield per tree, the average weight of the fruits and the diameter of the fruits. Based on our research, we made several observations: the pruning method did not affect the growth...
Nitrate/ammonium ratio effect on the growth, yield and foliar anatomy of grafted tomato plantsOriginal Paper
Armando Hernández Pérez, Juana Cruz García Santiago, Valentín Robledo Torres, Alonso Méndez López, Alberto Sandoval Rangel, Neymar Camposeco Montejo
Hort. Sci. (Prague), 2021, 48(2):80-89 | DOI: 10.17221/99/2020-HORTSCI
The vigorous behaviour of a rootstock modifies the growth and yield of a plant variety or hybrid, altering the plant nutritional requirements. The purpose of this work was to study four ratios of NO3-/NH4+ (100/0, 92/8, 85/15 and 80/20%) over the growth, leaf anatomy and yield of grafted and ungrafted tomato plants. We used a fully randomised experimental block design with factorial arrangement of 2 × 4 (grafted and ungrafted plants and four ratios of NO3-/NH4+), on eight treatments in total with four replicates each, using Tukey's mean comparison test (P ≤ 0.05). The rootstock was 'Silex' by Fito Seeds, with the 'El Arrojado' graft (variety)...
The effects of K+-deficiency on H2O2 dynamics and sucrose in tomatoOriginal Paper
Xiaoming Zhao, Ning Zhang, Xin Liu, Jing Jiang
Hort. Sci. (Prague), 2021, 48(2):90-97 | DOI: 10.17221/103/2020-HORTSCI
Potassium (K+) deficiency inhibits the transport of photosynthetic products and causes severe crop yield losses. However, the underlying mechanisms are poorly understood. In this study, we used two tomato lines 081018 (K+-deficiency-sensitive) and 081034 (K+-deficiency-tolerant), showing tolerance to K+ deficiency to investigate the relationship between the H2O2 and sucrose in the tomato under K+-deficiency. The H2O2 accumulation was increased by the low K+ condition (0.5 mM) after 8 h in 081018. The enzymes related to the metabolism of H2O2 were decreased, and more malondialdehyde (MDA) was produced. After...
Influence of Tytanit® and EM on biochemical, physiological, and qualitative parameters of common beanOriginal Paper
Alicja Auriga, Jacek Wróbel
Hort. Sci. (Prague), 2021, 48(2):98-104 | DOI: 10.17221/72/2020-HORTSCI
The role of preparations supporting plant growth is mainly to reduce the harmful effects of various stress factors on plants and to ensure high yields of good quality. This experiment compared the effect of the mineral stimulator Tytanit® and the biological preparation Effective Microorganisms (EM) on the physiological and biochemical activity, as well as the yield of the common bean (Phaseolus vulgaris L.). The photosynthetic pigments, free proline and malondialdehyde were assayed and compared at three phenological phases of the bean: 15 BBCH, 24 BBCH, 65 BBCH. The yield parameters included the average number of pods per plant, as well as their...