Hort. Sci. (Prague), 2024, 51(3):212-218 | DOI: 10.17221/90/2023-HORTSCI

Assessment of eggplant germplasm genetic diversity using RAPD markersOriginal Paper

Jelena Damnjanović1, Zdenka Girek2, Svetlana Roljević Nikolić ORCID...1, Milan Ugrinović3, Danica Mićanović4, Suzana Pavlović ORCID...2
1 Research and Development Institute Tamiš, Pančevo, Serbia
2 Institute for Medical Research, National Institute of Republic of Serbia, University of Belgrade, Belgrade, Serbia
3 Institute for Vegetable Crops Smederevska Palanka, Smederevska Palanka, Serbia
4 Serbian Chamber of Commerce and Industry, Bеlgrade, Serbia

Evaluation of genetic resources of eggplant (Solanum melongena L.) from different geographical areas using molecular markers (RAPD) is of great importance in the breeding process. A total of 90 polymorphic amplified products were obtained from 10 decametric RAPD primers, used to analyse the genetic diversity of 20 genotypes of eggplant (16 local and 4 genotypes of foreign origin). The highest polymorphism was determined using the OPAF-16 primer (70.83%). The number of detected bands ranged from 13 (OPF-04) to 24 (OPAF-16), while the average number of bands per primer was 17.2. The lengths of the amplified fragments ranged from 400 to 9 000 bp. The value of the Jaccard’s genetic distance coefficient ranged from 0.095 to 0.35, and the dendrogram constructed using the UPGMA method showed that 16 local and 4 foreign genotypes were grouped into nine groups (clusters). Populations K1, K8/1, K19, K22, K25 and K38 represent genotypes that separated from the others and formed single clusters. The lowest value of the calculated genetic distance was 0.095 between domestic genotypes K13 and K12, which also showed morphological similarity in terms of shape and colour. On the other hand, the highest value of genetic distance was calculated between foreign genotypes K19 and K25 (0.35), K19 and K34 (0.34) and K19 and K38 (0.34). Genetically distinct genotypes identified using RAPD markers could be potential starting genetic material for crossing with other genotypes to obtain new and improved eggplant varieties.

Keywords: Solanum melongena L., genetic resources, diversity, RAPD markers

Received: August 14, 2023; Revised: December 19, 2023; Accepted: March 7, 2024; Prepublished online: September 23, 2024; Published: September 29, 2024  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Damnjanović J, Girek Z, Roljević Nikolić S, Ugrinović M, Mićanović D, Pavlović S. Assessment of eggplant germplasm genetic diversity using RAPD markers. Hort. Sci. (Prague). 2024;51(3):212-218. doi: 10.17221/90/2023-HORTSCI.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Caguiat X.G.I., Hautea D.M. (2014): Genetic diversity analysis of eggplant (Solanum melongena L.) and related wild species in the Philippines using morphological and SSR markers. SABRAO Journal of Breeding and Genetics, 46: 183-201.
    2. Demir K., Bakir M., Sarikamiş G., Acunalp S. (2010): Genetic diversity of eggplant (Solanum melongena) germplasm from Turkey assessed by SSR and RAPD markers. Genetics and Molecular Research, 9: 1568-1576. Go to original source...
    3. Doganlar S., Frary A., Daunay C., Lester N., Tanskley S. (2002): A comparative genetic linkage map of eggplant (Solanum melongena L.) and its implication for genome evolution in the Solanaceae. Genetics, 161: 1697-1711. Go to original source... Go to PubMed...
    4. Fallah A.A., Sarmast E., Fatehi P., Jafari T. (2020): Impact of dietary anthocyanins on systemic and vascular inflammation: Systematic review and meta-analysis on randomized clinical trials. Food and Chemical Toxicology, 135: 110922. Go to original source...
    5. FAOSTAT (2023): Available at https://www.fao.org/faostat/en/#data/QCL
    6. Im K., Lee J.Y., Byeon H., Hwang K.W., Kang W., Whang W.K., Min H. (2016): In vitro antioxidative and anti-inflammatory activities of the ethanol extract of eggplant (Solanum melongena) stalks in macrophage RAW 264.7 cells. Food and Agricultural Immunology, 27: 758-771. Go to original source...
    7. Isshiki S., Suzuki S., Yamashita K. (2003): RFLP analysis of mitochondrial DNA in eggplant and related Solanum species. Genetic Resources and Crop Evolution, 50: 133-137. Go to original source...
    8. Jaccard P. (1908): Nouvelles recherchers sur la distribution florale. Bulletin de la Société Vaudoise des Sciences Naturelles, 44: 233-270.
    9. Kashyap V., Vinod K.S., Collonnier C., Fusari F., Haicour R., Rotino G.L., Sihachakr D., Rajam M.V. (2003): Biotechnology of eggplant. Scientia Horticulturae, 97: 1-25. Go to original source...
    10. Kaur M., Singh S., Karihaloo J.L. (2004): Diversity of enzyme electrophoretic patterns in the eggplant complex. Journal of Plant Biochemistry and Biotechnology, 13: 69-72. Go to original source...
    11. Kumchai J., Wei Y.C., Lee C.Y., Chen F.C., Chin S.W. (2013): Production of interspecific hybrids between commercial cultivars of the eggplant (Solanum melongena L.) and its wild relative S. torvum. Genetics and Molecular Research, 12: 755-764. Go to original source...
    12. Moury B., Pflieger S., Blattes A., Lefebvre V., Palloix A. (2000): A CAPS marker to assist selection of tomato spotted wilt virus (TSWV) resistance in pepper. Genome, 43: 137-142. Go to original source...
    13. Muñoz-Falcón J.E., Vilanova S., Plazas M., Prohens J. (2011): Diversity, relationships, and genetic fingerprinting of the Listada de Gandia eggplant landrace using genomic SSRs and EST-SSRs. Scientia Horticulturae, 129: 238-246. Go to original source...
    14. Naeem M.Y., Ugur S. (2019): Nutritional content and health benefits of eggplant. Turkish Journal of Agriculture - Food Science and Technology, 7: 31-36. Go to original source...
    15. Noda Y. Kneyuki T., Igarashi K., Mori A., Packer L. (2000): Antioxidant activity of nasunin, an anthocyanin in eggplant peels. Toxicology, 148: 119-23. Go to original source... Go to PubMed...
    16. Nunome T., Negoro S., Kono I., Kanamori H., Miyatake K., Yamaguchi H., Ohyama A., Fukuoka H. (2009): Development of SSR markers derived from SSR-enriched genomic library of eggplant (Solanum melongena L.). Theoretical and Applied Genetics, 119: 1143-1153. Go to original source... Go to PubMed...
    17. Pavlović S., Damnjanović J., Girek Z., Belić L., Ugrinović M. (2024): Induction of direct somatic embryogenesis and genetic stability of somatic embryo-derived plants of broccoli. Czech Journal of Genetics and Plant Breeding, 60: 50-54. Go to original source...
    18. Prohens J., Rodríguez-Burruezo A., Raigón M.D., Nuez F. (2007): Total phenolic concentration and browning susceptibility in a collection of different varietal types and hybrids of eggplant: Implications for breeding for higher nutritional quality and reduced browning. Journal of the American Society for Horticultural Science, 132: 638-646. Go to original source...
    19. Sifau M.O., Akinpelu A., Ogunkanmi L.A., Adekoya K.O., Oboh B.O., Ogundipe O.T. (2014): Genetic diversity in Nigerian brinjal eggplant (Solanum melongena L.) as revealed by random amplified polymorphic DNA (RAPD) markers. African Journal of Biotechnology, 13: 2119-2126. Go to original source...
    20. Singh A.K., Singh M., Singh R., Kumar S., Kalloo G. (2006): Genetic diversity within the genus Solanum (Solanaceae) as revealed by RAPD markers. Current Science, 90: 711-716.
    21. Stommel J.R., Whitaker B.D. (2003): Phenolic acid content and composition of eggplant fruit in a germplasm core subset. Journal of the American Society for Horticultural Science, 128: 704-710. Go to original source...
    22. Sunseri F., Polignano G.B., Alba V., Lotti C., Bisignano V., Mennella G., Alessandro A.D., Bacchi M., Riccardi P., Fiore M.C., Ricciardi L. (2010): Genetic diversity and characterization of African eggplant germplasm collection. African Journal of Plant Science, 4: 231-241.
    23. Tajik N., Tajik M., Mack I., Enck P. (2017): The potential effects of chlorogenic acid, the main phenolic components in coffee, on health: a comprehensive review of the literature. European Journal of Nutrition, 56: 2215-2244. Go to original source... Go to PubMed...
    24. Tiwari A., Rajesh S.J., Piyush T., Nayak S. (2009): Phytochemical investigations of crown of Solanum melongena L. fruit. International Journal of Phytomedicine, 1: 9-11. Go to original source...
    25. Zhou X., Cao G., Lin R., Sun Y., Li W. (1994): A rapid and efficient DNA extraction method of genus Fagopyrum for RAPD analysis. In: Javornik B., Bohanec B., Kreft I. (eds): Proceedings of Impact of Plant Biotechnology on Agriculture. Biotechnical Faculty, 5-7 June, Ljubljana, Slovenia: 171-175.

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content