Hort. Sci. (Prague), 2022, 49(3):154-163 | DOI: 10.17221/67/2021-HORTSCI

Physiological status, rooting and bulb yield of leaf cuttings of Eucomis comosa (Houtt.) H.R. Wehrh. 'Sparkling Burgundy' as affected by chitosanOriginal Paper

Paulina Kukla, ¯urawik Piotr*
Department of Horticulture, West Pomeranian University of Technology in Szczecin, Szczecin, Poland

Breeding efforts within the Eucomis genus has resulted in the global availability of attractive cultivars with decorative leaves and inflorescences. A cultivar that is particularly valuable and attractive, but rarely cultivated due to its low propagation rate is 'Sparkling Burgundy'. In our research, we considered it advisable to check the possibility of intensifying the reproduction of the investigated cultivar by means of leaf cuttings. This study, conducted in the years 2016-2018, involved leaf cuttings collected from plants growing in an unheated polytunnel. The cuttings were soaked, watered or sprayed with 0.4% chitosan with a molecular weight of 7 000 or 10 000 g/mol. The control plants were not treated with chitosan. During the rooting period that followed, the cuttings were assessed for their chlorophyll a fluorescence and the intensity of their greenness, and for their root growth and bulb yield after five months. Chitosan applied during rooting improved some chlorophyll fluorescence parameters and the greenness index of the cuttings. Moreover, the compound with a molecular weight of 7 000 g/mol more efficiently increased the number and length of the roots and the number and weight of the adventitious bulbs than that with a molecular weight of 10 000 g/mol. Soaking the cuttings prior to rooting was more effective in terms of the number, length and weight of the produced roots and the number, diameter and weight of the daughter bulbs than watering or spraying.

Keywords: adventitious rooting; senescence; bulb reproduction; cutting necrosis

Published: September 30, 2022  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Kukla P, Piotr ¯. Physiological status, rooting and bulb yield of leaf cuttings of Eucomis comosa (Houtt.) H.R. Wehrh. 'Sparkling Burgundy' as affected by chitosan. Hort. Sci. (Prague). 2022;49(3):154-163. doi: 10.17221/67/2021-HORTSCI.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Algam S.A.E., Xie G., Li B., Yu S., Su T., Larsen J. (2010): Effects of paenibacillus strains on plant growth promotion and control Ralstonia wilt in tomato. Journal of Plant Pathology, 92: 593-600.
    2. Angelini G., Ragni P., Esposito D., Giardi P., Pompili M.L., Moscardelli R., Giardi M.T. (2001): A device to study the effect of space radiation on photosynthesic organizms. Physica Medica, 17: 267-268.
    3. Baker N.R., Rosenqvist E. (2004): Application of chlorophyll fluorescence can improve crop production strategies: an examination of future possibilities. Journal of Experimental Botany, 55: 1607-1621. Go to original source... Go to PubMed...
    4. Bautista-Baños S., Hernández-Lauzardo A.N., Velázquez-Del Valle M.G., Hernández-López M., Ait Barka E., BosquezMolina E., Wilson C.L. (2006): Chitosan as a potential natural compound to control pre and postharvest diseases of horticultural commodities. Crop Protection, 25: 108-118. Go to original source...
    5. Carlson A.S., Dole J.M. (2015): Determining optimal bulb storage and production methods for successful forcing of cut pineapple lily. HortTechnology, 25: 608-616. Go to original source...
    6. Cheesman L., Finnie J., Van Staden J. (2010): Eucomis zambesiaca baker: Factors affecting in vitro bulbet induction. South African Journal of Botany, 76: 534-549. Go to original source...
    7. Coqueiro D.S.O., di Piero R.M. (2011): Antibiotic activity against Xanthomonas gardneri and protection of tomato plants by chitosan. Journal of Plant Pathology, 93: 337-344.
    8. Druege U. (2020): Overcoming physiological bottlenecks of leaf vitality and root development in cuttings: a systemic perspective. Frontiers in Plant Science, 11: 907. Go to original source... Go to PubMed...
    9. Duncan G. (2011): Eucomis vandermerwei. Curtis's Botanical Magazine, 28: 176-189. Go to original source...
    10. Dzung N.A., Khanh V.T.P., Dzung T.T. (2011): Research on impact of chitosan oligomers on biophysical characteristics, growth, development and drought resistance of coffee. Carbohydrate Polymers, 84: 751-755. Go to original source...
    11. El-Tanahy A.M.M., Mahmoud A.R., Abde-Mouty M.M., Ali A.H. (2012): Effect of Chitosan doses and nitrogen sources on the growth, yield and seed quality of cowpea. Australian of Journal Basic and Applied Sciences, 6: 115-121.
    12. Górnik K., Grzesik M., Romanowska-Duda B. (2008): The effect of chitosan on rooting of grapevine cuttings and on subsequent plant growth under drought andtemperature stress. Journal of Fruit and Ornamental Plant Research, 16: 333-343.
    13. Gregorczyk A., Raczyñska A., Pacewicz K. (1998): Analiza krzywych wzorcowych zawarto¶ci chlorofilu dla podstawowych gatunków zbó¿. Biuletyn Magnezologiczny, 3: 19-24.
    14. Hadwiger L.A., Klosterman S.J., Choi J.J. (2002): The mode of action of chitosan and its oligomers in inducing plant promoters and developing disease resistance in plants. Advances in Chitin Science, Bangkok, 5: 452-457.
    15. Hasegawa A., Kanechika R. (2005): Effect of low temperature and chitosan on dormancy breaking and growth of young corms of three Arisaema species. Acta Horticulturae (ISHS), 673: 603-609. Go to original source...
    16. Kaczmarek-Cichosz R., Chojnacki J., Deszcz E. (2011): Evaluation of efficiency of use of Biochikol 020 PC to semi-dry spring wheat grain dressing. Journal of Research and Applications in Agricultural Engineering, 56: 184-188.
    17. Kalaji M.H., £oboda T. (2010): Fluorescencja chlorofilu w badaniach stanu fizjologicznego ro¶lin. Wydawnictwo SGGW, 29-52.
    18. Kananont N., Pichyangkura R., Chanprame S., Chadchawan S., Limpanavech S. (2010): Chitosan specificity for the in vitro seed germination of two Dendrobium orchids (Asparagales: Orchidaceae). Scientia Horticulturae, 124: 239-247. Go to original source...
    19. Kapczyñska A. (2019): Propagation of Lachenalia cultivars from leaf cuttings. Acta Scientiarum Polonorum Hortorum Cultus, 18: 189-195. Go to original source...
    20. Kariuki S. (2008): Typhoid fever in sub-Saharan Africa: challenges of diagnosis and management of infections. The Journal of Infection in Developing Countries, 2: 443-447. Go to original source... Go to PubMed...
    21. Knippels P.J.M. (2000): Propagation of Speciality Bulbs. Herbertia, 55: 64-73.
    22. Knippels P.J.M. (2012): Advanced in vitro propagation Techniques for Speciality Bulbs. Floriculture and Ornamental Biotechnology, 6: 154-157.
    23. Lichtenthaler H.K., Buschmann C., Knapp M. (2005): How to correctly determine the different chlorophyll fluorescence parameters and the chlorophyll fluorescence decrease ratio RFd of leaves with the PAM fluorometer. Photosynthetica, 43: 379-393. Go to original source...
    24. Limpanavech P., Chaiyasuta S., Vongpromek R., Pichyangkura R., Khunwasi C., Chadchawan S., Latrakul P., Bunjongrat R., Chaidee A., Bangyeekhun T. (2008): Chitosan effects on floral production, gene expression, and anatomical changes in the Dendrobium orchid. Scientia Horticulturae, 116: 65-72. Go to original source...
    25. Lizárraga-Paulín E.G., Torres-Pacheco I., Moreno-Martínez E., Miranda-Castro S.P. (2011): Chitosan application in maize (Zea mays) to counteract the effects of abiotic stress at seedling level. African Journal of Biotechnology, 10: 6439-6446.
    26. Mahdavi B., Sanavy S.A.M.M., Aghaalikhani M., Sharifi M., Dolatabadian A. (2011): Chitosan improves osmotic potential tolerance in safflower (Carthamnus tinctorius) seedlings. Journal of Crop Improvement, 25: 728-741. Go to original source...
    27. Masondo N.A., Finnie J.F., Van Staden J. (2014): Pharmacological potential and conservation prospect of the genus Eucomis (Hyacinthaceae) endemic to southern Africa. Journal of Ethnopharmacology, 151: 44-53. Go to original source... Go to PubMed...
    28. Mondal M.M.A., Malek M.A., Puteh A.B., Ismail M.R., Ashrafuzzaman M., Naher L. (2012): Effect of foliar application of chitosan on growth and yield in okra. Australian Journal of Crop Science, 6: 918-921.
    29. Ndhlala A.R., Mulaudzi R.B., Kulkarni M.G., Van Staden J. (2012): Effect of environmental factors on seedling growth, bulb development and pharmacological properties of medicinal Eucomis autumnalis. South African Journal of Botany, 79: 1-8. Go to original source...
    30. Nge K.L., Nwe N., Chandrkrachang S., Stevens W.F. (2006): Chitosan as growth stimulator in orchid tissue culture. Plant Science, 170: 1185-1190. Go to original source...
    31. Niekraszewicz A., Wi¶niewska-Wrona M., Kopania E., Orlikowski L.B., Pospieszny H. (2012): Biopolymer compositions for ecological protection and growth stimulation of plants. Progress of Chemistry and Application of Chitin and its Derivatives, 145-158.
    32. Nndwambi D., Matsiliza-Mlathi B., Kleynhans R. (2013): Optimizing in vivo bulblet formation on leaf cuttings in Eucomis spp. South African Journal of Botany, 86: 179. Go to original source...
    33. Obsuwan K., Sawangsri K., Uthairatanakij A. (2010): Influence of foliar chitosan sprays on growth of mokara and phalaenopsis seedlings. Acta Horticulturae (ISHS), 867: 295-302. Go to original source...
    34. Peng S., Krieg D.R., Girma F.S. (1991): Leaf photosynthetic rate is correlated with biomass and grain production in grain sorghum lines. Photosynthesis Research, 28: 1-7. Go to original source... Go to PubMed...
    35. Pichyangkura R., Chadchawan S. (2015): Biostimulant activity of chitosan in horticulture. Scientia Horticulturae, 196: 49-65. Go to original source...
    36. Ramos-Garcia M., Ortega-Centeno S., Hernández-Lazuardo A.N., Alia-Tejacal I., Bosquez-Molina E., Bautista-Baños S. (2009): Response of gladiolus (Gladiolus spp.) plants after exposure corms to chitosan and hot water treatments. Scientia Horticulturae, 121 : 480-484. Go to original source...
    37. Reigossa R. M. J., Weiss O. (2001): Fluorescence techniques. In: Reigosa R.M. 9th Ed. Handbook of Plant Ecophysiology Techniques. Academic Publishers, Dordrecht, The Netherlands: 155-171.
    38. Rinaudo M. (2006): Chitin and chitosan: properties and applications. Progress in Polymer Science, 31: 603-632. Go to original source...
    39. Salachna P., Bartkowiak A., Kamiñska M., MazurkiewiczZapa³owicz K. (2008): Wp³yw miejsca uprawy i chitozanu o ró¿nym ciê¿arze cz±steczkowym na wzrost i plonowanie frezji odmiany 'Lisa'. Czê¶æ II. Plon i zdrowotno¶æ bulw. Zeszyty Problemowe Postêpów Nauk Rolniczych, 525: 375-382.
    40. Salachna P., Skierkowska M. (2010): Wp³yw masy sadzonki dwu³uskowej i chitozanu na plon cebul przybyszowych eucharisu wielkokwiatowego (Eucharis × grandiflora Planch. Et Linden). Zeszyty Problemowe Postêpów Nauk Rolniczych, 551: 321-326.
    41. Salachna P., Zawadziñska A. (2014a): Optimization of Ornithogalum saundersiae baker propagation by twin scale cuttings with the use of biopolymers. Journal of Basic and Applied Sciences, 10: 514-518. Go to original source...
    42. Salachna P., Zawadziñska A. (2014b): Effect of chitosan on plant growth, flowering and corms yield of potted freesia. Journal of Ecological Engineering, 15: 97-102.
    43. Salachna P., Wilas J., Zawadziñska A. (2015a): The effect of chitosan coating of bulbs on the growth and flowering of Ornithogalum saundersiae. Acta Horticulturae (ISHS), 1104: 115-118. Go to original source...
    44. Salachna P., Zawadziñska P., Wilas J. (2015b): The use of natural polysaccharides in Eucomis autumnalis (Mill.) Chitt. propagation by twin-scale cuttings. Acta Horticulturae (ISHS), 1104: 225-227. Go to original source...
    45. Sheikha S.A.A.K., AL-Malki F.M. (2011): Growth and chlorophyll responses of bean plants to the chitosan applications. European Journal of Scientific Research, 50: 124-134.
    46. Startek L., Bartkowiak A., Salachna P., Kamiñska M., Mazurkiweicz-Zapa³owicz K. (2005): The influence of New methods of corm coating on freesia growth, development and health. Acta Horticulturae (ISHS), 673: 611-616. Go to original source...
    47. Stirbet A, Govindjee. (2011): On the relation between the Kautsky effect (chlorophyll a fluorescence induction) and photosystem II: basics and applications of the OJIP fluorescence transient. Journal of Photochemistry and Photobiology B: Biology, 104: 236-57. Go to original source... Go to PubMed...
    48. ¦migielska M., Jerzy M. (2013): Adventitious roots and bulbs formation on Hyacinthus orientalis leaf cuttings under different colours of artificial light. Acta Scientiarum Polonorum. Hortorum Cultus, 12: 157-164.
    49. Taylor J.L.S., van Staden J. (2001): The effect of age, season and growth conditions on antiinflammatory activity in Eucomis autumnalis (Mill.) Chitt. Plant extracts. Plant Growth Regulation, 34: 39-47. Go to original source...
    50. Uddin A.F.M., Hashimoto F., Shimizu K., Sakata Y. (2004): Monosaccharides and chitosan sensing in bud growth and petal pigmentation in Eustoma grandiflorum (Raf.) Shinn. Scientia Horticulturae, 100: 127-138. Go to original source...
    51. Yang F., Hu J., Li J., Wu X., Qian Y. (2009): Chitosan enhances leaf membrane stability and antioxidant enzyme activites in apple seedlings under drought stress. Plant Growth Regulation, 58: 131-136. Go to original source...
    52. ¯urawik P. (2013): The impact of dried shrimp waste and chitosan as well as of the methods of cultivation on growth, development, decorative value and yield of cormlets of freesia (Freesia Eckl. ex Klatt). West Pomeranian University of Technology in Szczecin, Poland: 1-128.
    53. ¯urawik P., ¯urawik A., Kukla P., Dobrowolska A. (2017): Morphological traits, decorative value and yield of corms of freesia (Freesia Eckl. ex Klatt) depending on the applied chitosan. Acta Scientiarum Polonorum Hortorum Cultus, 16: 73-83.

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content