Hort. Sci. (Prague), 2022, 49(1):48-51 | DOI: 10.17221/68/2020-HORTSCI

Effect of activated charcoal and ascorbic acid on in vitro morphogenesis and o-dihydroxyphenols content in Paphiopedilum insigneShort Communication

Monika Poniewozik, Marzena Parzymies ORCID...*, Paweł Szot
Institute of Horticultural Production, Faculty of Horticulture and Landscape Architecture, University of Life Sciences in Lublin, Lublin, Poland

Phenolic compounds limit micropropagation of many orchids in vitro. The aim of the study was to estimate the effect of activated charcoal (AC);1, 2 or 4 g/L) or ascorbic acid (AA; 10, 20 or 30 mg/L) added to the half strength MS medium on the growth and o-dihydroxyphenols content in Paphiopedilum insigne in vitro. A positive effect of AC on the shoot and root formation has been found. The highest multiplication rate (5.6 shoots/explant) and rooting frequency were obtained on medium containing 2 g/L of AC. However, AC reduced the leaf number as compared to the control. The lowest content of o-dihydroxyphenols was marked in Paphiopedilum insigne leaves when the shoots were grown on medium with 10 mg/L AA, followed by AC at 1 or 2 g/L.

Keywords: lady slipper; orchid; micropropagation; phenolic compounds; tissue culture

Published: March 12, 2022  Show citation

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Poniewozik M, Parzymies M, Szot P. Effect of activated charcoal and ascorbic acid on in vitro morphogenesis and o-dihydroxyphenols content in Paphiopedilum insigne. Hort. Sci. (Prague). 2022;49(1):48-51. doi: 10.17221/68/2020-HORTSCI.
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