Hort. Sci. (Prague), 2022, 49(1):1-9 | DOI: 10.17221/51/2020-HORTSCI

Resolving the browning during the establishment of the in vitro propogation of Prunus avium cv. 'Fuchen'Original Paper

Yinkai Xi1,2, Biao Zeng3, Hengyu Huang*,1,4, Ye Wang*,1,5, Pinhui Yang1
1 Yunnan Breeding and Cultivation Research and Development Center of Endangered and Daodi Chinese Medicinal Materials, Yunnan University of Chinese Medicine, Kunming, P.R. China
2 Chemistry Department, Guizhou University of Traditional Chinese Medicine, Guiyang, P.R. China
3 Medicinal Plants Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, P.R. China
4 Qiucheng Breeding Company Limited, Lijiang, P.R. China
5 Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, P.R. China

Prunus avium cv. 'Fuchen' is a cultivated species with a high-profile economic benefit. Four media were used as a basal media equipped with four exogenous hormones to establish an intact regeneration system. Sodium thiosulfate (Na2S2O3) and active carbon (AC) improved the browning phenomenon. The results indicated that 1.0 g/L of AC and 30 g/L of Na2S2O3 were the best combination to inhibit the browning on the optimal basal medium - olive medium (OM) (followed by woody plant medium). The OM containing 1.5 mg/L of zeatin, 2.0 mg/L of α-naphtalenaecetic acid (NAA) and 0.5 mg/L of kinetin was beneficial for the germination and growth of the axillary buds. Green bud points were visible at the base of the swollen base after 25 days with a high proliferation coefficient (more than 7.50). The most optimal medium for rooting was 1/2 OM combined with 1.0 mg/L of NAA and 0.5 mg/L of indole-3-butyric-acid with a 100% rooting rate. The survival rate was up to 100% after 60 days with acclimatisation. Generally, a high-effective regeneration system was established, which provides a reference for keeping the excellent traits of the cultivar.

Keywords: sweet cherry 'Fuchen'; bud proliferation; basal medium; rapid propagation; browning inhibition

Published: March 12, 2022  Show citation

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Xi Y, Zeng B, Huang H, Wang Y, Yang P. Resolving the browning during the establishment of the in vitro propogation of Prunus avium cv. 'Fuchen'. Hort. Sci. (Prague). 2022;49(1):1-9. doi: 10.17221/51/2020-HORTSCI.
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