Hort. Sci. (Prague), 2021, 48(3):134-140 | DOI: 10.17221/142/2020-HORTSCI

Storage of proliferating gooseberry cultures under slow growth conditionsShort Communication

Danuta Kucharska ORCID..., Teresa Orlikowska, Robert Maciorowski, Małgorzata Kunka, Angelika Niewiadomska-Wnuk
Research Institute of Horticulture, Skierniewice, Poland

Short storage of in vitro cultures under slow-growth conditions is included in the commercial large-scale micropropagation process. It is dictated by the organizational scheme that provides temporary stop multiplication of shoots for some months. To avoid subculturing to fresh media every 4 weeks, which is obligatory for gooseberry, they can be kept in conditions that protect them from ageing, by slowing down their metabolism. To develop a rational schedule of gooseberry micropropagation, two experiments were used to adopt a temperature and length of time for storage. The best results were obtained with storage conditions at 2 °C for two or four months for proliferating cultures. Under these conditions, the percentage of necrotic shoots was low (< 10%), and shoot proliferation in the subsequent passages was at a level similar to proliferation cultures incubated in the growth room and sub-cultured monthly. The rate of shoots > 1 cm was higher than in the control in the growth room. Storage at 4 °C increased the probability of necrotic shoots up to 80% and decreased the number of all shoots and shoots > 1 cm in subsequent passages.

Keywords: Ribes grossularia L.; micropropagation; necrotic explants; storage

Published: September 30, 2021  Show citation

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Kucharska D, Orlikowska T, Maciorowski R, Kunka M, Niewiadomska-Wnuk A. Storage of proliferating gooseberry cultures under slow growth conditions. Hort. Sci. (Prague). 2021;48(3):134-140. doi: 10.17221/142/2020-HORTSCI.
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    References

    1. Aitken-Christie J., Singh A.P. (1987): Cold storage of tissue cultures. In: Bonga J.M., Durzan D.J. (eds): Tissue Culture in Forestry. The Hague, Martinus Nijhof/Dr. W. Junk: 285-304. Go to original source...
    2. Borkowska B. (1986): Dormancy of micropropagated sour cherry plantlets. Tree Physiology, 1: 303-307. Go to original source... Go to PubMed...
    3. Brennan R.M., Millam S., Davidson D., Wilshin A. (1990): Establishment of an in vitro Ribes germplasm collection and preliminary investigations into long-term low temperature germplasm storage. Acta Horticulturae (ISHS), 280: 110-112. Go to original source...
    4. Caplin S.M. (1959): Mineral oil for conservation of plant tissue culture. American Journal of Botany, 46: 324-329. Go to original source...
    5. Cha-um S., Kirdmanee C. (2007): Minimal growth in vitro culture for preservation of plant species. Fruit, Vegetable and Cereal Science and Biotechnology, 1: 13-25.
    6. Debnath S.C. (2003): Micropropagation of small fruits. In: Jain S.M., Ishii E. (eds): Micropropagation of Woody Trees and Fruits. Springer Science+Business Media, Dordrecht: 465-506. Go to original source...
    7. De Lacerda L.F., Gomes H.T., Bartos P.M.C., Vasconcelos J.M., Filho S.C.V., Silva-Cardoso I.M.A., Scherwinski-Pereira J.E. (2020): Growth, anatomy and histochemistry of fast growing species under in vitro conservation through mineral oil and low-temperature combination. Plant Cell, Tissue and Organ Culture (PCTOC), 144: 143-156. Go to original source...
    8. Drew R.A., Smith M.K. (1990): Field evaluation of tissuecultured bananas in south-eastern Queensland. Australian Journal of Experimental Agriculture, 30: 569-574. Go to original source...
    9. Dubois L.A.M., Roggermans J., Soyeurt G., de Vries D.P. (1988): Comparison of the growth and development of dwarf rose cultivars propagated in vitro and in vivo by softwood cuttings. Sciencia Horticulturae, 55: 293-299. Go to original source...
    10. Engelman F. (2011): Use of biotechnologies for the conservation of plant biodiversity. In Vitro Cellular & Developmental Biology - Plant, 47: 5-16. Go to original source...
    11. Gunning J., Lagerstedt H.B. (1985): Long-term storage techniques for in vitro plant germplasm. International Plant Propagators' Society, 35: 199-205.
    12. Howard B.H., Jones O.P., Vasek J. (1989): Long-term improvement in the rooting of plum cuttings following apparent rejuvenation. Journal of Horticultural Science, 64: 147-156. Go to original source...
    13. James R.J.E. (1980): Machine for harvesting soft fruit. US Patent, 4, 186, 547, Feb., 1980.
    14. Jones O.P. (1994): Physiological change and apparent rejuvenation of temperate fruit trees from micropropagation. In: Lumsden P.J., Nocholas J.R., Davies W.J. (eds): Physiology, Growth and Development of Plants in Culture. Dordrecht, Springer Science+Business Media: 323-331. Go to original source...
    15. Jones O.P., Vine S.J. (1968): The culture of gooseberry shoot tips for eliminating virus. Journal of Horticultural Science, 43: 289-292. Go to original source...
    16. Kucharska D., Orlikowska T., Maciorowski R., Kunka M., Wójcik D., Pluta S. (2020): Application of meta-topolin for improving micropropagation of gooseberry (Ribes grossularia). Sciencia Horticulturae, 272: 109529. Go to original source...
    17. Lisek A., Orlikowska T. (2008): The post-effect of in vitro storage on shoot multiplication and rooting Senga Sengana strawberry shoots. Journal of Fruit and Ornamental Plant Research, 16: 123-132.
    18. Mullin R.H., Schlegel D.E. (1962): Cold storage maintenance of strawberry meristem plantlets. HortScience, 11: 100-101 Go to original source...
    19. Murashige T., Skoog F. (1962): A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiologia Plantarum, 15: 473-497. Go to original source...
    20. Orlikowska T. (1992): Effect of in vitro storage at 4 °C on survival and proliferation of two apple rootstocks. Plant Cell, Tissue and Organ Culture (PCTOC), 31: 1-7. Go to original source...
    21. Pluta S. (2018): Gooseberry - Ribes uva-crispa, sin. R. grossularia L. In: Exotic Fruits. Academic Press: 211-218. Go to original source...
    22. Reed B.M., Chang Y. (1997): Chapter 4. Medium and longterm storage of in vitro cultures of temperate fruit and nut crops. In: Razdan M.K., Cocking E.C. (eds): Conservation of Plant Genetic Resources In Vitro. M/S Science Publishers, Inc., USA., 1: 67-105
    23. Reed B.M., Hummer K.E. (2002): Cryopreservation of Ribes. In: Towill L.E., Bajaj Y.P.S. (eds): Biotechnology in Agriculture and Forestry. Heidelberg, Springer Verlag Berlin: 323-343. Go to original source...
    24. Wang P.J., Hu C.Y. (1980): Regeneration of virus-free plants through in vitro culture. In: Fiechter A. (ed.): Advances in Biomedical Engineering, Vol. 18. Heidelberg, Springer Verlag, Berlin: 61-99. Go to original source...
    25. Welander M. (1985): Micropropagation of gooseberry, Ribes grossularia. Scientia Horticulturae, 26: 267-272. Go to original source...
    26. Wójcik D., Trzewik A., Kucharska D. (2021): Field performance and genetic stability of micropropagated gooseberry plants (Ribes grossularia L.). Agronomy 2021, 11: 45. Go to original source...

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