Hort. Sci. (Prague), 2020, 47(3):158-168 | DOI: 10.17221/81/2018-HORTSCI

Physio-biochemical responses of sage genotypes to chillingOriginal Paper

Andrzej Kalisz*,1, Agnieszka Sękara1, Robert Pokluda2, Aleą Jezdinský2, Jarmila Neugebauerová2, Aneta Grabowska1, Rita Jurkow1, Katalin Angéla Slezák3
1 Department of Horticulture, University of Agriculture in Krakow, Kraków, Poland
2 Department of Vegetable Sciences and Floriculture, Mendel University in Brno, Lednice, Czech Republic
3 Department of Vegetable and Mushroom Growing, Szent István University, Budapest, Hungary

This study evaluated sage (Salvia officinalis L.) genotypes (cultivars: 'Berggarten', 'Icterina', 'Purpurascens', 'Tricolor', local Czech accessions from the Lednice region, South Moravia: 'LDN-1' and 'LDN-2') subjected to chilling (4 °C, 2 weeks, 18 °C ‒ control) for comparison of antioxidant defence systems. Chilling caused the most significant increase in the peroxidase activity in 'Purpurascens' and 'Tricolor', by 108.5% and 15.7%, respectively, while the catalase was unaffected by the low temperature. The phenolics increased in 'Purpurascens' and 'LDN-1' by 17.2% and 18.1%, respectively, and decreased in 'LDN-2' and 'Tricolor', by 10.6% and 11.7%, respectively, as a result of the chilling. In the sage treated with chilling, the scavenging of 2,2-diphenyl-1-picrylhydrazyl radicals (DPPH*) was higher (by 3%, on average), especially in 'Berggarten', 'Icterina', and 'Purpurascens', than in the control. However, the chilled 'LDN-2' and 'Tricolor' showed lower antioxidant∙ activity in comparison to the control. The malondialdehyde remained stable or was higher in the control, with the only exception being 'LDN-1', where its content increased by 11.4% in the chilled sage. In most genotypes, the content of the dry weight increased in the chilled plants by 9.4% on average. The responses of 'Icterina' and 'Purpurascens' to the low temperature was the most significant, but resulted from different physiological mechanisms. 'Purpurascens' showed the highest increase in the peroxidase activity due to the chilling, while the highest increase in the antioxidant activity was observed for 'Icterina'.

Keywords: antioxidants; genotypic variability; Salvia officinalis; low temperature

Published: September 30, 2020  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Kalisz A, Sękara A, Pokluda R, Jezdinský A, Neugebauerová J, Grabowska A, et al.. Physio-biochemical responses of sage genotypes to chilling. Hort. Sci. (Prague). 2020;47(3):158-168. doi: 10.17221/81/2018-HORTSCI.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Abid M., Abid Ali Khan M.M. (2019): Medicinal plant: Environment interaction and mitigation to abiotic stres. In: Egamberdieva D., Tiezzi A. (eds): Medically Important Plant Biomes: Source of Secondary Metabolites. Singapore, Springer Nature Singapore Pte Ltd.: 21-50. Go to original source...
    2. Aebi H. (1984): Catalase in vitro. Methods in Enzymology, 105: 121-126. Go to original source... Go to PubMed...
    3. Afonso A.F., Pereira O.R., Fernandes Â., Calhelha R.C., Silva A.M.S., Ferreira I.C.F.R., Cardoso S.M. (2019): Phytochemical composition and bioactive effects of Salvia africana, Salvia officinalis 'Icterina' and Salvia mexicana aqueous extracts. Molecules, 24: 4327 Go to original source... Go to PubMed...
    4. B±czek-Kwinta R., Serek B., W±tor A. (2007): Effect of chilling on total antioxidant capacity and growth processes of basil (Ocimum basilicum L.) cultivars. Herba Polonica, 53: 75-84.
    5. Dhindsa R.S., Matowe W. (1981): Drought tolerance in two mosses: correlated with enzymatic defense against lipid peroxidation. Journal of Experimental Botany, 32: 79-91. Go to original source...
    6. Caverzan A., Casassola A., Brammer S.P. (2016): Reactive oxygen species and antioxidant enzymes involved in plant tolerance to stress. In: Shanker A. (ed.): Abiotic and Biotic Stress in Plants ‒ Recent Advances and Future Perspectives. London, UK, InTechOpen: 463-480. Go to original source...
    7. Cuceu A.V.P., Tofană M., Socaci S.A., Nagy M., Borș M.-D., Salanță L., Vlaic R. (2015): Studies on total polyphenols content and antioxidant activity of methanolic extracts from selected Salvia species. Bulletin UASVM Food Science and Technology, 72: 86-90. Go to original source...
    8. Cuvelier M.E., Richard H., Berset C. (1996): Antioxidative activity and phenolic composition of pilot-plant and commercial extracts of sage and rosemary. Journal of the American Oil Chemists' Society, 73: 645. Go to original source...
    9. Djeridane A., Yousfi M., Nadjemi B., Boutassouna D., Stocker P., Vidal N. (2006): Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compound. Food Chemistry, 97: 654-660. Go to original source...
    10. Gill S.S., Tuteja N. (2010): Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry, 48: 909-930. Go to original source... Go to PubMed...
    11. Guo W.L., Chen R.G., Gong Z.H., Yin Y.X., Ahmed S.S., He Y.N. (2012): Exogenous abscisic acid increases antioxidant enzymes and related gene expression in pepper (Capsicum annuum) leaves subjected to chilling stress. Genetics and Molecular Research, 11: 4063-4080. Go to original source... Go to PubMed...
    12. Guo Z., Ou W., Lu S., Zhong Q. (2006): Differential responses of antioxidative system to chilling and drought in four rice cultivars differing in sensitivity. Plant Physiology and Biochemistry, 44: 828-836. Go to original source... Go to PubMed...
    13. Hasanuzzaman M, Borhannuddin Bhuyan MHM, Anee TI, Parvin K, Nahar K, Al Mahmud J, Fujita M. (2019): Regulation of ascorbate-glutathione pathway in mitigating oxidative damage in plants under abiotic stress. Antioxidants, 8: 384. Go to original source... Go to PubMed...
    14. Kalisz A., Pokluda R., Jezdinský A., Sękara A., Grabowska A., Gil J., Neugebauerová J. (2016): Chilling-induced changes in the antioxidant status of basil plants. Acta Physiologiae Plantarum, 38: 196. Go to original source...
    15. Kanase T., Guhey A., Gawas D. (2019): Activity of antioxidant enzymes in soybean genotypes under drought stress. International Journal of Current Microbiology and Applied Sciences, 8: 2323-2330. Go to original source...
    16. Kang H.-M., Saltveit M.E. (2002): Effect of chilling on antioxidant enzymes and DPPH-radical scavenging activity of high- and low-vigour cucumber seedling radicles. Plant, Cell & Environment, 25: 1233-1238. Go to original source...
    17. Karpinsky S., Wingsle G., Karpinska B., Hällgren J.-E. (2002): Low temperature stress and antioxidant mechanisms to oxidative stress in plants. In: Inzé D., Van Montagu M. (eds): Oxidative Stress in Plants. London, UK, Taylor & Francis: 85-128.
    18. Karuppanapandian T., Moon J.-C., Kim C., Manoharan K., Kim W. (2011): Reactive oxygen species in plants: their generation, signal transduction, and scavenging mechanisms. Australian Journal of Crop Science, 5: 709-725.
    19. Kuk Y.I., Shin J.S., Burgos N.R., Hwang T.E., Han O., Cho B.H., Jung S., Guh J.O. (2003): Antioxidative enzymes offer protection from chilling damage in rice plants. Crop Science, 43: 2109-2117. Go to original source...
    20. Kusvuran S., Kiran S., Ellialtioglu S.S. (2016): Antioxidant enzyme activities and abiotic stress tolerance relationship in vegetable crops. In: Shanker A. (ed.): Abiotic and Biotic Stress in Plants - Recent Advances and Future Perspectives. London, UK, InTechOpen: 481-506. Go to original source...
    21. Lakuąić B.S., Ristić M.S., Slavkovska V.N., Stojanović D.L., Lakuąić D.V. (2013): Variations in essential oil yields and compositions of Salvia officinalis (Lamiaceae) at different developmental stages. Botanica Serbica, 37: 127-139.
    22. Lee D.H., Lee C.B. (2000): Chilling stress-induced changes of antioxidant enzymes in the leaves of cucumber: in gel enzyme activity assays. Plant Science, 159: 75-85. Go to original source... Go to PubMed...
    23. Lee J.-H., Oh M.-M. (2015): Short-term low temperature increases phenolic antioxidant levels in kale. Horticulture, Environment, and Biotechnology, 56: 588-596. Go to original source...
    24. Lu J., Nawaz M.A., Wei N., Cheng F., Bie Z. (2020): Suboptimal temperature acclimation enhances chilling tolerance by improving photosynthetic adaptability and osmoregulation ability in watermelon. Horticultural Plant Journal, 6: 49-60. Go to original source...
    25. Lu Y., Foo L.Y. (2002): Polyphenolics of Salvia - a review. Phytochemistry, 59, 117-140. Go to original source... Go to PubMed...
    26. Lück H. (1962): Peroxidase. In: Bergmeyer H.U. (ed.): Methoden der enzymatischen analyse. Weinheim, Germany, Verlag Chemie GmbH: 895-897.
    27. Molyneux P. (2004): The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology, 26: 211-219.
    28. Naikoo M.I., Dar M.I., Raghib F., Jaleel H., Ahmad B., Raina A., Khan F.A., Naushin F. (2019): Role and regulation of plants phenolics in abiotic stress tolerance: An overview. In: Khan M.I.R., Reddy P.S., Ferrante A., Khan N.A. (eds): Plant Signaling Molecules, Role and Regulation Under Stressful Environments. Elsevier: 157-168. Go to original source...
    29. Oh M.M., Carey E.E., Rajashekar C.B. (2009): Environmental stresses induce health-promoting phytochemicals in lettuce. Plant Physiology and Biochemistry, 47: 578-583. Go to original source... Go to PubMed...
    30. Pijanowski E., Mrożewski S., Horubała A. (1964): Technologia produktów owocowych i warzywnych [Technology of Fruit and Vegetable Products]. Warszawa, Poland, PWRiL.
    31. Pohl A., Komorowska M., Kalisz A., Sękara A. (2019): Eggplant seedlings modify antioxidant system during acclima-tion to low temperature. Agrochimica, 62: 151-167. Go to original source...
    32. Posmyk M.M., Bailly Ch., Szafrańska K., Janas K.M., Corbineau F. (2005): Antioxidant enzymes and isoflavonoids in chilled soybean (Glycine max (L.) Merr.) seedlings. Journal of Plant Physiology, 162: 403-412. Go to original source... Go to PubMed...
    33. Poulios E., Giaginis C., Vasios G.K. (2020): Current state of the art on the antioxidant activity of sage (Salvia spp.) and its bioactive components. Planta Medica, 86: 224-238. Go to original source... Go to PubMed...
    34. Rezaie R., Mandoulakani B.A., Fattahi M. (2020): Cold stress changes antioxidant defense system, phenylpropanoid contents and expression of genes involved in their biosynthesis in Ocimum basilicum L. Scientific Reports, 10: 5290. Go to original source... Go to PubMed...
    35. Rivero R.M., Ruiz J.M., García P.C., López-Lefebre L.R., Sánchez E., Romero R. (2001): Resistance to cold and heat stress: accumulation of phenolic compounds in tomato and watermelon plants. Plant Science, 160: 315-321. Go to original source... Go to PubMed...
    36. Rodríguez V.M., Soengas P., Alonso-Villaverde V., Sotelo T., Cartea M.E., Velasco P. (2015): Effect of temperature stress on the early vegetative development of Brassica oleracea L. BMC Plant Biology, 15: 145. Go to original source... Go to PubMed...
    37. Ruelland E., Collin S. (2012): Chilling stress. In: Shabala Ş. (ed.): Plant Stress Physiology. CAB International: 94-117. Go to original source...
    38. Sharma P., Jha A.B., Dubey R.S., Pessarakli M. (2012): Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. Journal of Botany, 2012: 217037. Go to original source...
    39. Shen W., Nada K., Tachibana S. (1999): Effect of cold treatment on enzymic and nonenzymic antioxidant activities in leaves of chilling-tolerant and chilling-sensitive cucumber cultivars. Journal of the Japanese Society for Horticultural Science, 68: 967-973. Go to original source...
    40. Sivaci A., Kaya A., Duman S. (2014): Effects of ascorbic acid on some physiological changes of pepino (Solanum muricatum Ait.) under chilling stress. Acta Biologica Hungarica, 65: 305-318. Go to original source... Go to PubMed...
    41. Suzuki N., Mittler R. (2006): Reactive oxygen species and temperature stresses: A delicate balance between signaling and destruction. Physiologia Plantarum, 126: 45-51. Go to original source...
    42. Szentmihályi K., Then M., Csedö C. (2004): Comparative study on tannins, flavonoids, terpenes and mineral elements of some Salvia species. Acta Horticulturae (ISHS), 629: 463-470. Go to original source...
    43. Wojdyło A., Oszmiański J., Czemerys R. (2007): Antioxidant activity and phenolic compounds in 32 selected herbs. Food Chemistry, 105: 940-949. Go to original source...
    44. Xu S.-C., Li Y.-P., Hu J., Guan Y.-J., Ma W.-G., Zheng Y.-Y, Zhu S.-J. (2010): Responses of antioxidant enzymes to chilling stress in tobacco seedlings. Agricultural Sciences in China, 9: 1594-1601. Go to original source...
    45. You J., Chan Z. (2015): ROS regulation during abiotic stress responses in crop plants. Frontiers in Plant Science, 6: 1092. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content