Hort. Sci. (Prague), 2019, 46(2):90-97 | DOI: 10.17221/239/2017-HORTSCI

Cryopreservation of Caninae rose shoot tips guarantees biochemical stability and pollination potential monitored in four-year-old regenerantsOriginal Paper

Bożena Pawłowska*,1, Jarmila Neugebauerová2, Monika Bieniasz3, Bożena Szewczyk-Taranek1, Ivo Ondrasek4
1 Department of Ornamental Plants, University of Agriculture in Krakow, Kraków, Poland
2 Department of Vegetable Growing and Floriculture, Mendel University in Brno, Lednice, Czech Republic
3 Department of Pomology and Apiculture, University of Agriculture in Krakow, Kraków, Poland
4 Department of Fruit Growing, Mendel University in Brno, Lednice, Czech Republic

Stability of Rosa agrestis, R. canina, R. dumalis and R. rubiginosa regenerants after shoot tip cryopreservation was monitored with the use of biochemical markers and pollen quality assessment in matured plants cultivated in the field. The results of rosa pollen and hip evaluation revealed no significant differences between post-cryopreserved regenerants and control plants; however, variations in the assessed markers were noted between Rosa species. Analyses of hips confirmed high levels of vitamin C in these plants; the highest was observed in R. dumalis (750-870 mg/100 g fresh weight). High total antioxidant activity (TAC) was as follows: DPPH - 50-250 mM TE/100 dry weight, FRAP - 80-180 mM TE/100 g dry weight. The present study showed a high content of flavonoids (TFC) and phenols (TPC). Post-cryopreserved plants retained pollen specific for Caninae: numerous anthers, abundant pollen, poor pollen germination and viability and typical pollen morphology. This study demonstrates new tools for evaluating the fidelity of post-cryopreserved regenerants.

Keywords: hypanthia; pollen; TAC; TFC; TPC; vitamin C

Published: June 30, 2019  Show citation

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Pawłowska B, Neugebauerová J, Bieniasz M, Szewczyk-Taranek B, Ondrasek I. Cryopreservation of Caninae rose shoot tips guarantees biochemical stability and pollination potential monitored in four-year-old regenerants. Hort. Sci. (Prague). 2019;46(2):90-97. doi: 10.17221/239/2017-HORTSCI.
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    References

    1. Alexander M.P. (1969): Differential staining of aborted and nonaborted pollen. Journal of Stain Technology, 44: 117-122. Go to original source... Go to PubMed...
    2. Alp S., Ercisli S., Jurikova T., Cakir O., Gozlekci S. (2016): Bioactive content of rose hips of different wildly grown Rosa dumalis genotypes. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 44: 472-476. Go to original source...
    3. Arya S.P., Mahajan M., Jain P. (2000): Non-spectrophotometric methods for the determination of vitamin C. Analytica Chimica Acta, 417: 1-14. Go to original source...
    4. Bi W.L., Pan C., Liu J., Wang Q.C. (2016): Greenhouse performance, genetic stability and biochemical compounds in Chrysanthemum morifolium 'Hangju' plants regenerated form cryopreserved shoot tips. Acta Physiologia Plantarum, 38: 38-268. Go to original source...
    5. Bieniasz M., Necas T., Dziedzic E., Ondrasek I., Pawłowska B. (2017): Evaluation of pollen quality and self-fertility in selected cultivars of Asian and European pears. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 45: 375-382. Go to original source...
    6. Chrubasik C., Roufogalis B. D., Müller-Ladner U., Chrubasik S. (2008): A systematic review on the Rosa canina effect and efficacy profiles. Phytotherapy Research, 22: 725-733. Go to original source... Go to PubMed...
    7. Ercisli S. (2007a): Determination of pollen viability and in vitro pollen germination of Rosa dumalis and Rosa villosa. Bangladesh Journal of Botany, 36: 185-187. Go to original source...
    8. Ercisli S. (2007b): Chemical composition of fruits in some rose (Rosa spp.) species. Food Chemistry, 104: 1379-1384. Go to original source...
    9. Fatemi N., Attar F., Assareh M.H., Hamzehee B. (2012): Pollen morphology of the genus Rosa L. (Rosaceae) in Iran. Iran Journal of Botany, 18: 284-293.
    10. Harding K. (2004): Genetic integrity of cryopreserved plant cells: a review. CryoLetters, 25: 3-22.
    11. Kaity A., Ashmore S.E., Drew R.A. (2009): Field performance evaluation and genetic integrity assessment of cryopreserved papaya clones. Plant Cell Report, 28: 1421-1430. Go to original source... Go to PubMed...
    12. Kaity A., Drew R.A., Ashmore S.E. (2013): Genetic and epigenetic integrity assessment of acclimatised papaya plants regenerated directly from shoot-tips following short- and long-term cryopreservation. Plant Cell Tissue and Organ Culture, 112: 75-86. Go to original source...
    13. Kulus D., Mikuła A. (2016): Cryopreservation in securing the stability and physiological activity of plant cells. Acta Scientiarum Polonorum Biotechnologia, 15: 27-38 (in Polish).
    14. Lachman J., Hamouz K., Čepl J., Pivec V., ©ulc M., Dvořák P. (2006): Vliv vybraných faktoru na obsah polyfenolu a antioxidační aktivitu hlíz brambor. Chemické listy, 100: 522-527.
    15. Martin C., Kremer C., Gonzalez I., Gonzalez-Benito M.E. (2015): Influence of the cryopreservation technique, recovery medium and genotype on genetic stability of mint cryopreserved shoot tips. Plant Cell Tissue and Organ Culture, 122: 185-195. Go to original source...
    16. Nybom H., Werlemark G., Esselink D.G., Vosman B. (2005): Sexual preferences linked to Rose taxonomy and cytology. Acta Horticulturae (ISHS), 690: 21-28. Go to original source...
    17. Nybom H., Werlemark G. (2017): Realizing the potential of health-promoting rosehips form dogroses (Rosa sec. Caninae). Current Bioactive Compounds, 13: 3-17. Go to original source...
    18. Pawłowska B., Szewczyk-Taranek B. (2014): Droplet vitrification cryopreservation of Rosa canina and Rosa rubiginosa using shoot tips from in situ plants. Scientia Horticulturae, 168: 151-156 Go to original source...
    19. Pawłowska B., Szewczyk-Taranek B. (2015): Efficient cryopreservation by droplet vitrification of pentaploid roses and the phenotype of regenerated plants. Acta Societatis Botanicorum Poloniae, 84: 439-442. Go to original source...
    20. Quave C.L., Pieroni A. (2015): A reservoir of ethnobotanics knowledge informs resilient food security and health strategies in the Balkans. Nature Plants: 14021. doi: 10.1038/nplants.2014.21 Go to original source... Go to PubMed...
    21. Roman I., Stanila A., Stanila S. (2013): Bioactive compounds and antioxidant activity of Rosa canina L. biotypes from spontaneous flora of Transylvania. Chemistry Central Journal, 7: 73. Go to original source... Go to PubMed...
    22. Ruľić DJ., Vujović T., Cerović R. (2014): Cryopreservation of cherry rootstock Gisela 5 using vitrification procedure. Horticultural Science (Prague), 41: 55-63. Go to original source...
    23. Shan B., Yizhong Z.C., Mei S., Corke H. (2005): Antioxidant capacity of 26 spice extracts and characterization of their phenolic constituents. Journal of Agricultural and Food Chemistry, 53: 7749-7759. Go to original source... Go to PubMed...
    24. Sharma N., Singh R., Pandey R., Kaushik N. (2017): Genetic and biochemical stability assessment of plants regenerated from cryopreserved shoot tips of commercially valuable medicinal herb Bacopa monnieri (L.) Wettst. In Vitro Cellular and Developmental Biology - Plant, 53: 346-351. Go to original source...
    25. Skyba M., Urbanowa M., Kapchina-Toteva V., Kosuth J., Harding K., Hellerova E. (2010): Physiological, biochemical and molecular characteristics of cryopreservated Hyperium perforatum L. shoot tips. CryoLetters, 31: 249-260.
    26. Stanila A., Diaconeasa Z., Roman I., Sima N., Maniutiu D., Roman A., Sima R. (2015): Extraction and characterization of phenolic compounds form rose hip (Rosa canina L.) using liquid chromatography coupled with electrospray ionization - mass spectrometry. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 43: 349-354. Go to original source...
    27. Taneva I., Petkova N., Dimov I., Ivan I., Denev P. (2016): Chracterization of rose hips (Rosa canina L.) fruits extracts and evaluation of their in vitro antioxidant activity. Journal of Pharmacognosy and Phytochemistry, 5: 35-38
    28. Wrońska-Pilarek D., Tomlik-Wyremblewska A. (2010): Pollen viability and in vitro germination of selected Central European species from genus Rosa analysed with different methods. Dendrobiology, 64: 43-53.
    29. Wrońska-Pilarek D. (2011): Pollen morphology of Polish native species of the rosa genus (Rosaceae) and its relation to systematic. Acta Societatis Botanicorum Poloniae, 80: 221-232. Go to original source...
    30. Yordanowa Z.P., Dimitrova M.A., Čellárová E., KapchinToteva V.M. (2011): Physiological evaluation of Hypericum rumeliacum Boiss. Plants regenerated after cryopreservation. Cryopreservation Symposium, COST Action 871 "Cryopreservation of crop species in Europe". Final meeting. Angers, France, February 8-11: 56-59
    31. Zloch Z., Čelakovský J., Aujezdská A. (2004): Stanovení obsahu polyfenolu a celkové antioxidační kapacity v potravinách rostlinného původu. Závěrečná zpráva o plnění projektu podpořeného finančně Nadačním fondem Institutu Danone, Plzeň.
    32. Zhang Z., Skjeseth G., Elameen A., Haugslien S., Silvertsen A., Clarke J.L., Wang Q.-Ch., Blystad D.-R. (2015): Field performance evaluation and genetic integrity assessment in Argyranthemum 'Yellow Empire' plant srecovered from cryopreserved shoot tips. In Vitro Cellular and Developmental Biology-Plants, 51: 505-513. Go to original source...

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