Hort. Sci. (Prague), 2017, 44(4):186-194 | DOI: 10.17221/266/2015-HORTSCI

An efficient in vitro propagation protocol for snowdrop anemone (Anemone sylvestris L.)Original Paper

Jana Šedivá*,1, Pavla Zahumenická2, Eloy Fernández Cusimamani*,2
1 Department of Phytoenergy and Biodiversity, Silva Tarouca Research Institute for Landscape and Ornamental Gardening, Průhonice, Czech Republic
2 Department of Crop Sciences and Agroforestry, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Prague, Czech Republic

This study investigated in vitro production of diploid (AS2) and tetraploid (AS4) cytotypes of snowdrop anemone. The effect of plant growth regulators (PGRs) on in vitro shoot multiplication and rooting was investigated. The effect of activated charcoal (AC) on root induction was also studied. Ploidy level affected growth characteristics during multiplication and rooting. Shoot induction in AS4 was higher on medium supplemented with cytokinin (3.2-3.6), while the AS2 clone formed the most shoots on PGR-free medium (3.6). The highest rooting percentage was achieved on PGR-free medium in both genotypes (AS2 clone, 100% and AS4 clone, 93.3%). The addition of AC to the PGR media largely increased root induction and root length. Rooted plantlets were successfully acclimatised in the greenhouse with 100% survival. Thus, the described micropropagation protocol represents a rapid and effective in vitro propagation method for utilisation in horticulture and conservation programmes of snowdrop anemone.

Keywords: Ranunculaceae; tetraploid; rhizogenesis; shoot organogenesis; activated charcoal

Published: December 31, 2017  Show citation

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Šedivá J, Zahumenická P, Fernández Cusimamani E. An efficient in vitro propagation protocol for snowdrop anemone (Anemone sylvestris L.). Hort. Sci. (Prague). 2017;44(4):186-194. doi: 10.17221/266/2015-HORTSCI.
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