Hort. Sci. (Prague), 2015, 42(3):114-118 | DOI: 10.17221/352/2014-HORTSCI

Molecular analysis of native cultivars of sweet cherry in Southern ItalyOriginal Paper

C. Di Vaio, C. Villano, N. Marallo
Department of Agricultural Sciences, University of Naples Federico II, Portici, Italy

Campania region has a long tradition of autochthonous cultivated sweet cherries, which constitute a very rich germplasm resource. This biodiversity is highly valued for flavour, aroma and pulp texture. The interested cultivars are well known and sold in regional and local markets, but rarely outside. Genetic studies and phenotypic classifications are useful tools to increase our knowledge of such cherry cultivars and to disseminate their particular characteristics outside the region. The aim of the present study was the molecular characterization of ten autochthonous cultivars of Prunus avium using 30 RAPD markers. Among all, U17 was useful for fingerprinting eight out of ten cultivars. On cvs Del Monte, Della Recca, Pagliaccio, Montenero, Nera Dura, Mulegnana Nera, Passaguai and Malizia, unique molecular profiles were obtained. Furthermore, it was possible to distinguish between two most important cultivars on the Campanian market (cvs Del Monte and Della Recca) with HAP18 marker. The results obtained in this study confirmed the power of RAPD markers to easily analyse genetic diversity and to find new molecular profiles in a very short time. Moreover, confidential bands, characteristics of Campania native cultivars, can be used for genotype identification.

Keywords: Prunus avium; RAPD; genetic diversity; fingerprinting; biodiversity

Published: September 30, 2015  Show citation

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Di Vaio C, Villano C, Marallo N. Molecular analysis of native cultivars of sweet cherry in Southern Italy. Hort. Sci. (Prague). 2015;42(3):114-118. doi: 10.17221/352/2014-HORTSCI.
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