Hort. Sci. (Prague), 2014, 41(2):95-99 | DOI: 10.17221/266/2013-HORTSCI

In vitro elimination of Black raspberry necrosis virus from black raspberry (Rubus occidentalis) - Short CommunicationShort Communication

E.J. Cheong, A.R. Jeon, J.W. Kang, R. Mock, G. Kinard, R. Li
National Germplasm Resources Laboratory, Agricultural Research Service, United States Department of Agriculture, Beltsville, USA

Black raspberry necrosis virus (BRNV) is one of the most important viral pathogens of Rubus spp. In this study, a procedure combining in vitro culture and heat therapy on axillary buds of Rubusoccidentalis was developed to eliminate BRNV from infected plants. Axillary buds were grown aseptically at 4-h alternating periods of 29°C and 38°C with 14-h day length for 1-5 weeks. Shoots induced after thermotherapy were tested for the viruses by RT-PCR. BRNV was not detected in any shoots indicating that BRNV was heat labile. BRNV-free shoots were rooted in vitro and acclimatized in greenhouse. Regenerated plants remained virus-free after 2-month acclimatization, 3-month dormancy and 5-month growth periods.

Keywords: BRNV; Rubus occidentalis; axillary bud culture; thermotherapy

Published: June 30, 2014  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Cheong EJ, Jeon AR, Kang JW, Mock R, Kinard G, Li R. In vitro elimination of Black raspberry necrosis virus from black raspberry (Rubus occidentalis) - Short Communication. Hort. Sci. (Prague). 2014;41(2):95-99. doi: 10.17221/266/2013-HORTSCI.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Baumann G., 1982. Elimination of a heat-stable raspberry virus by combining heat treatment and meristem culture. Acta Horticulturae (ISHS), 129: 11-12. Go to original source...
    2. Cadman C.H., 1961. Raspberry viruses and virus diseases in Britain. Horticulture Researches, 1: 47-61.
    3. Chellappan P., Vanitharani R., Ogbe F., Fauquet C.M., 2005. Effect of temperature on geminivirus-induced RNA silencing in plants. Plant Physiology, 138: 1828-1841. Go to original source... Go to PubMed...
    4. Cheong E.J., Mock R., Li R., 2012. Elimination of five viruses from sugarcane using in vitro culture of axillary buds and apical meristems. Plant Cell, Tissue and Organ Culture, 109: 439-445. Go to original source...
    5. Halgren A., Tzanetakis I.E., Martin R.R., 2007. Identification, characterization, and detection of Black raspberry necrosis virus. Phytopathology, 97: 44-50. Go to original source... Go to PubMed...
    6. Hosokawa M., Otake A., Ohishi K., Ueda E., Hayashi T., Yazawa S., 2004. Elimination of chrysanthemum stunt viroid from an infected chrysanthemum cultivar by shoot regeneration from a leaf primordium-free shoot apical meristem dome attached to a root tip. Plant Cell Reports, 22: 859-863. Go to original source... Go to PubMed...
    7. Jones A.T., 1982. Distinctions between three aphid borne latent viruses of raspberry. Acta Horticulturae (ISHS), 129: 41-48. Go to original source...
    8. Jones A.T, McGavin W.J., Geering A.D.W., Lockhart B.E.L., 2002. Identification of Rubus yellow net virus as a distinct badnavirus and its detection by PCR in Rubus species and in aphids. Annals of Applied Biology, 141: 1-10. Go to original source...
    9. Karesova R., Janeckova M., Paprstein F., 2002, Elimination of raspberry bushy dwarf virus from raspberry cv. 'Gatineau'. Acta Horticulturae (ISHS), 585: 359-632. Go to original source...
    10. Lankes C., 1995. Elimination of raspberry bushy dwarf virus. Acta Horticulturae (ISHS), 385: 70-74. Go to original source...
    11. Li R., Mock R., Huang Q., Abad J., Hartung J., Kinard G., 2008. A reliable and inexpensive method of nucleic acid extraction for the PCR-based detection of diverse plant pathogens. Journal of Virology Methods, 154: 48-55. Go to original source... Go to PubMed...
    12. Martin R.R., 2002. Virus diseases of rubus and strategies for their control. Acta Horticulturae (ISHS), 585: 265-270. Go to original source...
    13. McGavin W.J., McMenemy L.S., MacFarlane S.A., 2010. The complete sequence of a UK strain of black raspberrynecrosis virus. Archives of Virology, 155: 1897-1899. Go to original source... Go to PubMed...
    14. Mellor F.C., Stace-Smith R., 1978. Eradication of Rubus viruses. Canadian Agriculture, 23: 28-29.
    15. Mellor F.C., Stace-Smith R., 1980. A heat-stable black raspberry necrosis virus. Acta Horticulturae (ISHS), 95: 71-76. Go to original source...
    16. Murashige T., Skoog F., 1962. A Revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15: 473-497. Go to original source...
    17. Panattoni A., Triolo E., 2010. Susceptibility of grapevine viruses to thermotherapy on in vitro collection of Kober 5BB. Scientia Horticulturae, 125: 63-67. Go to original source...
    18. Pollack S., Perez A., 2006. Fruit and Tree Nuts Situation and Outlook Yearbook, Washington DC, United States Department of Agriculture.
    19. Schliephake E., 2010. Aphid resistance in raspberry and feeding behaviour of Amphorophora idaei. Journal of Plant Diseases and Protection, 117: 60-66. Go to original source...
    20. Sharma S., Singh B., Rani G., Zaidi A.A., Hallan V., Nagpal A., Virk G.S., 2008. In vitro production of Indian citrus ringspot virus (ICRSV) free Kinnow plants employing thermotherapy coupled with shoot tip grafting. Plant Cell, Tissue and Organ Culture, 92: 85-92. Go to original source...
    21. Stace-Smith R., 1955. Studies on Rubus virus diseases in British Columbia. III. Separation of components of raspberry mosaic. Canadian Journal of Botany, 34: 435-442. Go to original source...
    22. Susaimuthu J., Tzanetakis I.E., Gergerich R.C., Martin R.R., 2006. Yellow vein-affected blackberries and the presence of a novel Crinivirus. Plant Pathology, 55: 607-613. Go to original source...
    23. Susaimuthu J., Gergerich R.C., Bray M.M., Clay K.A., Clark J.R., Tzanetakis I.E., Martin R.R., 2007. Incidence and Ecology of Blackberry yellow vein associated virus. Plant Disease, 91: 809-813. Go to original source... Go to PubMed...
    24. Wang L., Wang G., Hong N., Tang R., Deng X., Zhang H., 2006. Effect of thermotherapy on elimination of apple stem grooving virus and apple chlorotic leaf spot virus for in vitro-cultured pear shoot tips. HortScience, 41: 729-732. Go to original source...
    25. Wang Q., Cuellar W.J., Rajamäki M.L., Hirata Y., Valkonen J.P.T., 2008. Combined thermotherapy and cryotherapy for efficient virus eradication: relation of virus distribution, subcelluar changes, cell survival ad viral RNA degradation in shoot tips. Molecular Plant Pathology, 9: 237-250. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content