Hort. Sci. (Prague), 2014, 41(2):55-63 | DOI: 10.17221/234/2013-HORTSCI

Cryopreservation of cherry rootstock Gisela 5 using vitrification procedureOriginal Paper

Dj. Ružić1, T. Vujović1, R. Cerović2
1 Fruit Research Institute, Čačak, Serbia
2 Innovation Center, Faculty of Technology and Metallurgy, University of Belgrade, Belgrade, Serbia

In vitro-grown shoot tips of Gisela 5 (Prunus cerasus × Prunus canescens) cherry rootstock were tested for regrowth after cryopreservation using vitrification technique. Explants were precultured in the dark at 23°C, in a liquid MS medium with a progressively increasing sucrose concentration (0.3 M for 15 h, then 0.7 M for 5 h), and subsequently loaded in a solution containing 2 M glycerol and 0.4 M sucrose for 20 minutes. Shoot tips were dehydrated at 0°C using either the original PVS2 or modified PVS2 solution (PVS A3 - 22.5% sucrose, 37.5% glycerol, 15% ethylene glycol and 15% DMSO) for 30, 40 and 50 minutes. The survival and regrowth of the cryopreserved shoot tips dehydrated with the original PVS2 solution ranged between 36-54% and 8-17%, respectively. However, the dehydration with the PVS A3 solution resulted in considerably higher survival rates (81-92%), as well as higher regrowth rates (39-56%) after cryopreservation. These results prove the feasibility of the PVS A3-based vitrification technique for a long-term storage of this genotype.

Keywords: Prunus cerasus × Prunus canescens; liquid nitrogen; vitrification solutions

Published: June 30, 2014  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Ružić D, Vujović T, Cerović R. Cryopreservation of cherry rootstock Gisela 5 using vitrification procedure. Hort. Sci. (Prague). 2014;41(2):55-63. doi: 10.17221/234/2013-HORTSCI.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. De Carlo A., Benelli C., Lambardi M., 2000. Development of a shoot-tip vitrification protocols and comparison with encapsulation-based procedures for plum (Prunus domestica L.) cryopreservation. CryoLetters, 21: 215-222.
    2. Engelmann F., 1991. In vitro conservation of horticultural species. Acta Horticulturae (ISHS), 298: 327-334. Go to original source...
    3. Engelmann F., 2004. Plant cryopreservation: progress and prospects. In Vitro Cellular & Developmental Biology Plant, 40: 427-433. Go to original source...
    4. Gi Cho E., Hor Y.I., Kim H.H., Rao V.R., Engelmann F., 2002. Cryopreservation of Citrus madarensis embryogenic axes by vitrification: importance of loading and tretment with vitrification solution. CryoLetters, 23: 317-324.
    5. Kaviani B., 2011. Conservation of plant genetic resources by cryopreservation. Australian Journal of Crop Science, 5: 778-800.
    6. Kim H.H., Lee Y.G., Shin D.J., Ko H.C., Gwag J.G., Cho E.G., Engelmann F., 2009. Development of alternative plant vitrification solutions in droplet-vitrification procedures. CryoLetters, 30: 320-334. Go to original source...
    7. Kryszczuk A., Keller J., Grübe M., Zimnoch-Guzowska E., 2006. Cryopreservation of potato (Solanum tuberosum L.) shoot tips using vitrification and droplet method. Journal of Food, Agriculture & Environment, 4: 196-200.
    8. Murashige T., Skoog F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologya Plantarum, 15: 473-497. Go to original source...
    9. Niino T., Tashiro K., Suzuki M., Ohuchi S., Magoshi J., Akihama T., 1997. Cryopreservation of in vitro grown shoot tips of cherry and sweet cherry by one-step vitrification. Scientia Horticulturae, 70: 155-163. Go to original source...
    10. Panis B., Lambardi M., 2005. Status of cryopreservation technologies in plants (crops and forest trees). In: International workshop -The Role of Biotechnology - for the Characterisation and Conservation of Crop, Forestry, Animal and Fishery Genetic Resources, Turin: 43-54.
    11. Reed B.M., Schumacher L., Dumet D., Benson E.E., 2005. Evaluation of a modified encapsulation-dehydration procedure in corporating sucrose pretretments for the cryopreservation of Ribes germplasm. In vitro Cellular and Developmental Biology Plant, 41: 431-436. Go to original source...
    12. Sakai A., Engelmann F., 2007. Vitrification, encapsulationvitrification and droplet-vitrification: a review. CryoLetters, 28: 151-172.
    13. Sakai A., Kobayashi S., Oiyama I., 1990. Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by vitrification. Plant Cell Reports, 9: 30-33. Go to original source... Go to PubMed...
    14. Shatnawi M.A., Shibli R., Qrunfleh I., Bataeineh K., Obeidat M., 2007. In vitro propagation and cryopreservation of Prunus avium using vitrification and encapsulation dehydration methods. Journal of Food, Agriculture & Environment, 5: 204-208.
    15. Uemura M., Minami A., Kawamura Y., 2009. Effect of low temperatures and cryoprotectants on plant plasma membranes. In: Programme and Abstracts 1st International Symposium on Cryopreservation in Horticultural Species, Leuven: 15.
    16. Vujović T., Ružić Dj., Cerović R., 2012. In vitro shoot multiplication as influenced by repeated subculturing of shoots of contemporary fruit rootstocks. Horticultural Science (Prague), 39: 101-107. Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content