Hort. Sci. (Prague), 2011, 38(2):49-53 | DOI: 10.17221/10/2010-HORTSCI

The elimination of Plum pox virus in plum cv. Bluefree and apricot cv. Hanita by chemotherapy of in vitro cultures

A. Hauptmanová, J. Polák
Department of Virology, Crop Research Institute, Prague-Ruzyně, Czech Republic

In vitro cultures of plum cv. Bluefree and apricot cv. Hanita infected with Plum pox virus (PPV) were used for the virus elimination by chemotherapy. Low ribavirin concentrations of 5 and 10 mg/l in Murashige-Skoog medium were applied in the treatment. Plum pox virus was completely eliminated by 5 mg/l of ribavirin in plum cv. Bluefree within twenty weeks and in apricot cv. Hanita in twelve weeks of the application. Plum pox virus was completely eliminated by 10 mg/l of ribavirin both in plum cv. Bluefree and apricot cv. Hanita within twelve weeks. The presence of PPV was not proved by RT-PCR. Clones of plum cv. Bluefree and apricot cv. Hanita were re-tested by RT-PCR one year after the termination of the ribavirin treatment and negative results confirmed the elimination of Plum pox virus.

Keywords: sharka; plant virus; chemotherapy; ribavirin; apricot; plum

Published: June 30, 2011  Show citation

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Hauptmanová A, Polák J. The elimination of Plum pox virus in plum cv. Bluefree and apricot cv. Hanita by chemotherapy of in vitro cultures. Hort. Sci. (Prague). 2011;38(2):49-53. doi: 10.17221/10/2010-HORTSCI.
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