Hort. Sci. (Prague), 2006, 33(4):158-162 | DOI: 10.17221/3756-HORTSCI

Accumulation of flavonoid compounds in flowering shoots of Achillea collina Becker ex. Rchb. Alba during flower development

K. Karlová

Department of Gene Bank, Research Institute of Crop Production Prague-Ruzyně, Workplace in Olomouc, Czech Republic

The objective of this paper was a screening of flavonoid content in Achillea collina Becker ex. Rchb. Alba flowering tops (Herba millefolii) and flowers (Flos millefolii). Ten developmental stages of plant (flowers and tops separately) - from the beginning of flower differentiation to ripe seed stage - were studied. The methanol extract of plant material was used for HPLC analysis. The flavones apigenin, luteolin, and their 7-O-glucosides were found as the main flavonoid constituents in all developmental stages of both flower and top drug. Apigenin and apigenin-7-O-glucoside contents have a similar accumulation tendency during ontogenesis - their contents fluently increased until the full flower phase and then they decreased. Maximal apigenin and apigenin-7-O-glucoside content was established to range between 0.6 and 0.7 mg/g in millefolii herba and 0.9 and 1.3 mg/g in millefolii flos. The luteolin content has the same tendency and also quantity as apigenin and the highest value at the full flowering stage was found even stronger. On the other hand, luteolin-7-O-glucoside has the highest content at the stage of small flower bud (about 1.0 mg/g). Its amount decreased later on and it increased to the second maximum at the full flower phase.

Keywords: Achillea collina; flavonoids; apigenin; luteolin; HPLC; ontogenesi

Published: December 31, 2006  Show citation