Hort. Sci. (Prague), 2005, 32(3):104-107 | DOI: 10.17221/3774-HORTSCI

Identification of sucrase activity in cell suspension and culture medium of melon

J. Stano1, K. Mičieta2, A. Barth3, M. Valšíková4, M. Fulmeková1, P. Matejka1, M. Varadínová1
1 Faculty of Pharmacy, Comenius University, Bratislava, Slovak Republic
2 Faculty of Natural Sciences, Comenius University, Bratislava, Slovak Republic
3 Institute of Biochemistry, M. Luther University, Halle/Saale, Federal Republic of Germany
4 Research Institute of Vegetables, Nové Zámky, Slovak Republic

The activity of (soluble acid) sucrase was detected in a culture medium of the cell suspension culture of watermelon (Citrullus vulgaris L.). A simple and rapid procedure for the identification and determination of extracellular sucrase from a culture medium of watermelon cell suspension cultures is described. Sucrose was used as a substrate for the determination of extracellular and intracellular activities of the enzyme. Intracellular activity was estimated from the cell suspension. The results show a 91.5-92.0% intracellular and 8.0-8.5% extracellular distribution of sucrase activity. The described method enables to carry out a rapid, simple and specific detection of extracellular sucrase in plants.

Keywords: intracellular sucrase; extracellular sucrase; watermelon

Published: September 30, 2005  Show citation

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Stano J, Mičieta K, Barth A, Valšíková M, Fulmeková M, Matejka P, Varadínová M. Identification of sucrase activity in cell suspension and culture medium of melon. Hort. Sci. (Prague). 2005;32(3):104-107. doi: 10.17221/3774-HORTSCI.
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