Hort. Sci. (Prague), 2005, 32(1):31-41 | DOI: 10.17221/3763-HORTSCI

Role of bioinoculants and auxin in development of salt tolerant Mentha arvensis

S. Kashyap, S. Sharma
Centre for Rural Development and Technology, Indian Institute of Technology, Hauz Khas, New Delhi, India

Pot experiments were conducted for the development of salt tolerant Mentha arvensis (Japanese mint, family: Labiatae) saplings involving bioinoculants, namely Arbuscular Mycorrhizal (AM) fungi, Azotobacter and an auxin - Indole Acetic Acid (IAA). The IAA and sodium chloride (NaCl) concentrations were standardized prior to the experiments. The 10-ppm IAA and 0.08% NaCl (w/v) were found to be optimum in combination with AM fungi and Azotobacter to increase all the growth parameters and microbial count in the rhizosphere. For development of salt tolerant saplings, the optimal concentration of IAA, along with AM fungi and Azotobacter in different combinations, was applied in pots. The saplings were irrigated regularly with 0.08% NaCl water. Although plant growth, AM infection percentage, AM spores/100 g soil and Azotobacter cells/g soil were affected by NaCl watering, the inoculation of both bioinoculants significantly enhanced survival percentage of saplings from 10 to 40% under salt stress. Maximum survival (40%) of saplings was found with IAA (10 ppm) + AM fungi + Azotobacter treatment.

Keywords: Mentha arvensis; in vivo; AM fungi; Azotobacter; IAA; NaCl stress

Published: March 31, 2005  Show citation

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Kashyap S, Sharma S. Role of bioinoculants and auxin in development of salt tolerant Mentha arvensis. Hort. Sci. (Prague). 2005;32(1):31-41. doi: 10.17221/3763-HORTSCI.
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References

  1. AL-KARAKI G.N., 2000. Growth of mycorrhizal tomato and mineral acquisition under salt stress. Mycorrhiza, 10: 51-54. Go to original source...
  2. AZCON R., AZCON-AGUILLAR C., BAREA J.M., 1978. Effects of plant hormones present in bacterial cultures on the formation and responses to VA endomycorrhiza. New Phytologist, 80: 359-364. Go to original source...
  3. BAREA J.M., AZCON-AGUILAR C., AZCON R., 1997. Interaction between mycorrhizal fungi and rhizosphere microorganisms within the context of sustainable soil-plant systems. In: GANGE A.C., BROWN V.K. (eds.), Multitrophic Interactions in Terrestrial Systems. 36th Symposium of the British Ecological Society. Cambridge, Blackwell Science: 65-77.
  4. BHAT S., MAHEHWARI P., KUMAR S., KUMAR A., 2002. Mentha species: In vitro regeneration and genetic transformation. Molecular Biology Today, 3: 11-23.
  5. CLAPPERTON M.J., REID D.M., 1992. Effects of low-concentration sulphur dioxide fumigation and vesicular-arbuscular mycorrhizas on 14C-partitioning in Phleum pratense L. New Phytologist, 120: 381-387. Go to original source...
  6. CRISTOFERI G., BRANZANTI B., ZOCCA A. 1985. Role of mycorrhizae on fruit rootstock rooting. In: GIANINAZZIPEARSON V., GIANINAZZI S. (eds.), Proceedings of the 1st European Symposium on Mycorrhizae. Dijon, Institut National de la Recherche Agronomique: 483-488.
  7. GERDEMANN J.W., NICOLSON T.H., 1963. Spores of mycorrhizal endogone species extracted from soils by wet sieving and decanting. Transactions of the British Mycological Society, 46: 235-244. Go to original source...
  8. GOGALA N., 1991. Regulation of mycorrhizal infection by hormonal factors produced by hosts and fungi. Experimentia, 47: 331-340. Go to original source...
  9. GRYNDLER M., HRSELOVA H., CHVATALOVA I., JANSA J., 1998. The effect of selected plant hormones on in vitro proliferation of hyphae of Glomus fistulosum. Biologia Plantarum, 41: 255-263. Go to original source...
  10. GUPTA SOOD S., 2003. Chemotactic response of plantgrowth-promoting bacteria towards roots of vesicular-arbuscular mycorrhizal tomato plants. FEMS Microbiology Ecology, 45: 219-227. Go to original source... Go to PubMed...
  11. HIRREL M.C., GERDEMANN J.W., 1980. Improved growth of onion and bell pepper in saline soils by two VAM fungi. Soil Science Society of America Journal, 44: 654-655. Go to original source...
  12. JINDAL V., ATWAL A., SEKHON B.S., SINGH R., 1995. Influence of NaCl salinity on some aspects of nitrogen assimilation and mineral composition in moong (Vigna radiata L.) plants in the presence and absence of mycorrhizal inoculation. Journal of Indian Chemistry Society, 42: 41-45.
  13. JUNIPER, S., ABBOTT, L.K., 1993. Vesicular-arbuscular mycorrhizas and soil salinity. Mycorrhiza, 4: 45-47. Go to original source...
  14. KALDORF M., LUDWIG-MULLER J., 2000. AM fungi might affect the root morphology of maize by increasing indole3-butyric acid biosynthesis. Physiologia Plantarum, 109: 58-67. Go to original source...
  15. KUKREJA K., ANAND R.C., NARULA N., 1995. Comparative performance of growth hormones on the proliferation of Indole acetic acid producer/non-producer soil isolate of Azotobacter chroococcum. Annales Biologiques, 11: 134-136.
  16. KUKREJA A.K., DHAWAN O.P., 2000. Somaclonal breeding for improvement of herb and essential oil yield in mints: a case study. Journal of Medicinal Aromatical Plant Science, 22: 431-442.
  17. KUMAR B., RAM P., SHARMA S., RANJAN V., 1999. Status of menthol mint (Mentha arvensis L.) cultivation in India: A survey report on Haryana and Punjab. Indian Perfumer, 43: 83-87.
  18. MAFFEI M., MUCCIARELLI M., 2003. Essential oil yield in peppermint/soybean strip intercropping. Field Crops Resource, 84: 229-240. Go to original source...
  19. McHUGHEN A., SWARTZ M., 1984. A tissue culture derived salt tolerant line of flax (Linum usitatissimum). Journal of Plant Physiology, 117: 109-117. Go to original source... Go to PubMed...
  20. McMILLEN B.G., JUNIPER S., ABBOTT L.K., 1998. Inhibition of hyphal growth of a vesicular- arbuscular mycorrhizal fungus in soil containing sodium chloride limits the spread of infection from spores. Soil Biology and Biochemistry, 30: 1639-1646. Go to original source...
  21. NELSON R., ACHAR P.N., 2001. Stimulation of growth and nutrient uptake by VAM fungi in Brassica oleracea var. capitata. Biologia Plantarum, 44: 277-281. Go to original source...
  22. PHILLIPS J.M., HAYMAN D.S., 1970. Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection. Transactions of the British Mycological Society, 55: 158-161. Go to original source...
  23. RUIZ-LOZANO J.M., AZCON R., 2000. Symbiotic efficiency and infectivity of an autochthonous arbuscular mycorrhizal Glomus sp. from saline soils and Glomus deserticola under salinity. Mycorrhiza, 10: 137-143. Go to original source...
  24. SCAGEL C.F., LINDERMAN R.G., 1998. Relationships between differential in vitro indole-acetic acid or ethylene production capacity by ectomycorrhizal fungi and conifer seedling responses in symbiosis. Symbiosis, 24: 13-24.
  25. SELVARAJ T., MANIVANNAN G., 1997. VA-mycorrhizal fungi in saline soils of Nagai Quaid-E-Milleth district, Tamil Nadu. Geobios, 23: 17-20.
  26. SMITH S.E., SMITH F.A., 1990. Transley review No. 20, Structure and function of the interfaces in biotrophic symbioses as they relate to nutrients transport. New Phytologist, 114: 1-38. Go to original source... Go to PubMed...
  27. STEIN A., FORTIN J.A., 1990. Enhanced rooting of Picea mariana cuttings by ectomycorrhizal fungi. Canadian Journal of Botany, 68: 468-470. Go to original source...
  28. SUBBA RAO N.S., 1982. Biofertilizers in Agriculture. New Delhi, Oxford and IBH Publishing Co. Pvt. Ltd: 182.
  29. VILLAR-ARTEAGA C., ZUNIGA-DAVILA D., 1997. Diversity de Azotobacter sp. in zone altoandina of the Peru. Proceedings of the Society for Experimental Biology and Medicine, 124: 874-878.
  30. YADAV J.S.P., 2000. Management of salt affected soils for sustainable production. In: GAWANDE S.P., BALI J.S., DAS D.C., SARKAR T.K., DAS D.K., NARAYANASWAMY G. (eds.), Advances in Land Resource Management for 21st Century. Soil Conservation Society of India, New Delhi: 253-264.

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