Hort. Sci. (Prague), 2005, 32(1):6-8 | DOI: 10.17221/3759-HORTSCI
The effect of carbon source on plant regeneration in tomato
- Division of Applied Genetics and Breeding, Research Institute of Plant Production Piešťany, Piešťany, Slovak Republic
The effect of different tomato cultivars and different sugar types (sucrose, glucose and maltose) and concentrations (1.0, 2.0 and 3.0%) on shoot regeneration from aseptically grown hypocotyl and cotyledon explants were studied. Among sugar types, sucrose at a concentration of 3.0% induced the highest number of shoots from both types of explants. In hypocotyl explants, cv. Premium showed the best regeneration capacity (0.23 shoots per explant), and in cotyledon explants, cv. Hana produced the maximal number of shoots (0.43 or 0.37 for media with 2.0% or 3.0% sucrose, respectively).
Keywords: in vitro culture; MS medium; sugars; organogenesis
Published: March 31, 2005 Show citation
References
- COSTA M.G.C., NOGUEIRA F.T.S., FIGUEIRA M.L., OTONI W.C., BROMMONSCHENKEL S.H., CECON P.R., 2000. Influence of the antibiotic timentin on plant regeneration of tomato (Lycopersicon esculentum Mill.) cultivars. Plant Cell Reports, 19: 327-332.
Go to original source... 
Go to PubMed... - CHEN H.Y., ZHANG J.H., ZHUANG T.M., ZHOU G.H., 1999. Studies on optimum hormone levels for tomato plant regeneration from hypocotyl explants cultured in vitro. Acta Agriculture Shanghai, 15: 26-29.
- EL-BAKRY A.A., 2002. Effects of genotype, growth regulators, carbon source, and pH on shoot induction and plant regeneration in tomato. In vitro Cellular and Developmental Biology - Plant, 38: 501-507. Go to original source...
- EVANS D.A., 1989. Somaclonal variation - genetic basic and breeding applications. Trends in Genetics, 5: 46-50. Go to original source... Go to PubMed...
- FRARY A., EARLE E.D., 1996. An examination of factors affecting the efficiency of Agrobacterium-mediated transformation of tomato. Plant Cell Reports, 16: 235-240. Go to original source...
- ICHIMURA K., ODA M., 1995. Stimulation of shoot regeneration from cotyledon segments of tomato (Lycopersicum esculentum Mill.) by agar and its extract. Journal of the Japanese Society of Horticultural Science, 64: 135-141. Go to original source...
- MURASHIGE T., SKOOG F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum, 15: 473-497. Go to original source...
- PERES L.E.P., MORGANTE P.G., VECCHI C., KRAUS J.E., VAN SLUYS M.A., 2001. Shoot regeneration capacity from roots and transgenic hairy roots of tomato cultivars and wild related species. Plant Cell Tissue Organ Culture, 65: 37-44. Go to original source...
- SCHNAPP S.R., PREECE J.E., 1986. In vitro growth reduction of tomato and carnation microplant. Plant Cell Tissue Organ Culture, 6: 3-8. Go to original source...
- SNEDECOR G.W., COCHRAN W.G., 1956. Statistical Methods 5th ed. Ames, Iowa, The Iowa State University Press: 367.
- VAN ROEKEL J.S.C., DAMM B., MELCHERS L.S., HOEKEMA A., 1993. Factors influencing transformation frequency of tomato (Lycopersicum esculentum). Plant Cell Reports, 12: 644-647. Go to original source... Go to PubMed...
- VENKATACHALAM P., GEETHA N., PRIYA P., RAJASEGER G., JAYABALAN N., 2000. High frequency plantlet regeneration from hypocotyl explants of tomato (Lycopersicum esculentum Mill.) via organogenesis. Plant Cell Biotechnology and Molecular Biology, 1: 95-100.
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