Hort. Sci. (Prague), 2004, 31(2):63-69 | DOI: 10.17221/3794-HORTSCI

Content of polyphenolic antioxidants and trans-resveratrol in grapes of different varieties of grapevine (Vitis vinifera L.)

J. Lachman, M. ©ulc, A. Hejtmánková, V. Pivec, M. Orsák
Faculty of Agronomy, Czech University of Agriculture in Prague, Prague, Czech Republic

Contents of polyphenolic antioxidants as total polyphenols and trans-resveratrol in grape musts, grape skins and seeds of twelve vine varieties from the harvests 2001 and 2002 were determined. Polyphenolic antioxidants of grapes are very effective in preventing cancer and cardiovascular diseases. Total polyphenolic content was determined spectrophotometrically with phenol Folin-Ciocalteau's reagent and trans-resveratrol content by the HPLC method. The average annual total polyphenol contents in the years 2001 and 2002 were 171 mg/land 324 mg/lin the must, 7,470 mg/l and 15,600 mg/kg DM in grape skins and 91,450 and 107,440 mg/kg DM in the seeds. The average annual trans-resveratrol values in the years 2001 and 2002 reached 0.817.10-2 mg/kg DM and 4.97.10-2 mg/kg DM in grape skins and 52.7.10-2 mg/kg DM and 4.63.10-2 mg/kg DM in the seeds and there was hardly any trans-resveratrol presence in the must. The results were evaluated statistically by Statgraphic and Anova programmes. Statistically significant differences in total polyphenols were found between analysed vintages, varieties and contents in seeds and grape skins. Regarding the trans-resveratrol content, statistically significant differences between vintages were found.

Keywords: grapevine; grapes; must; skins; seeds; polyphenolic antioxidants; trans-resveratrol; effect of varieties, vintages, plant part

Published: June 30, 2004  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Lachman J, ©ulc M, Hejtmánková A, Pivec V, Orsák M. Content of polyphenolic antioxidants and trans-resveratrol in grapes of different varieties of grapevine (Vitis vinifera L.). Hort. Sci. (Prague). 2004;31(2):63-69. doi: 10.17221/3794-HORTSCI.
Download citation

References

  1. ADRIAN M., RAJAEI H., JEANDET P., VENEAU J., BESSIS R., 1998. Resveratrol oxidation in Botrytis cinerea conidia. Phytopathology, 88: 472-476. Go to original source... Go to PubMed...
  2. BAVARESCO L., CANTU E., FREGONI M., TREVISAN M., 1997a. Constitutive stilbene contents of grapevine cluster stems as potential source of resveratrol in wine. Vitis, 36: 115-118.
  3. BAVARESCO L., PETEGOLLI D., CANTU E., FREGONI M., CHIUSA G., TREVISAN M., 1997b. Elicitation and accumulation of stilbene phytoalexins in grapevine berries infected by Botrytis cinerea. Vitis, 36: 77-83.
  4. BIANCHINI F., VAINIO H., 2003. Wine and resveratrol mechanisms of cancer prevention? Eur. J. Cancer Prevention, 12: 417-425. Go to original source... Go to PubMed...
  5. BORBALAN A.M.A., ZORRO L., GUILLEN D.A., BARROSO C.G., 2003. Study of the polyphenol content of red and white grape varieties by liquid chromatography - mass spectrometry and its relationship to antioxidant power. J. Chromatogr. A, 1012: 31-38. Go to original source... Go to PubMed...
  6. BURNS J., GARDNER P.T., O'NEIL J., CRAWFORD S., MORECROFT I., MC PHAIL D.B., LISTER C., MATTHEWS D., MAC LEAN M.R., DUTHIE G.G., CROZIER A., 2000. Relationship among antioxidant activity, vasodilation capacity, and phenolic content of red wines. J. Agr. Food Chem., 48: 220-230. Go to original source... Go to PubMed...
  7. CANTOS E., ESPIN J.C., TOMAS-BARBERAN F.A., 2002. Varietal differences among the polyphenol profiles of seven table grape cultivars studied by LC/DAD/MS/MS. J. Agric. Food Chem., 50: 5691-5696. Go to original source... Go to PubMed...
  8. CASTELLARI M., SPINABELLI U., RIPONI C., AMATI A., 1998. Influence of some technological practices on the quantity of resveratrol in wine. Z. Lebensm. Unters. Forsch. A, 206: 151-155. Go to original source...
  9. CHICON R.M., SANCHEZ-PALOMO E., CABEZUDO M.D., 2002. The colour and polyphenol composition of red wine varieties in Castilla - La Mancha (Spain). Afinidad, 59: 435-443.
  10. DE BEER D., JOUBERT E., GELDERBLOM W.C.A., MANLEY M., 2003. Antioxidant activity of South African red and white cultivar wines: Free radical scavenging. J. Agric. Food Chem., 51: 902-909. Go to original source... Go to PubMed...
  11. FILIP V., PLOCKOVÁ M., ©MIDRKAL J., ©PIČKOVÁ Z., MELZOCH K., SCHMIDT S., 2003. Resveratrol and its antioxidant and antimicrobial effectiveness. Food Chem., 83: 585-593. Go to original source...
  12. HERTOG M.G.L.,1998. Flavonols in wine and tea and prevention of coronary heart disease. In: VERCAUTEREN J., CHÈZE C., TRIAUD J. (eds.), Polyphenols 96, July 15-18, 1996, Bordeaux. Paris, INRA: 117-131.
  13. KENNEDY J.A., MATTHEWS M.A., WATERHOUSE A.L., 2002. Effect of maturity and vine water status on grape skin and wine flavonoids. Amer. J. Enol. Vitic., 53: 268-274. Go to original source...
  14. KONNEH M., CAEN J., 1998. Red wine derived compounds and their putative antiaherogenic properties. In: VERCAUTEREN J., CHÈZE C., TRIAUD J. (eds.), Polyphenols 96, July 15-18, 1996, Bordeaux. Paris, INRA: 105-115.
  15. KOPEC K., 1999. Resveratrol - chemoprotektivní sloľka vinných hroznů. Zahradnictví - Hort.. Sci. (Prague), 26: 135-138.
  16. LACHMAN J., HOSNEDL V., PIVEC V., ORSÁK M., 1998. Polyphenols in cereals and their positive and negative role in human and animal nutrition. Proc. of Cereals for Human Health and Preventive Nutrition, July 7-11, Brno: 118-125.
  17. MAKRIS D.P., PSARRA E., KALLITHRAKA S., KEFALAS P., 2003. The effect of polyphenolic composition as related to antioxidant capacity in white wines. Food Res. Int., 36: 805-814. Go to original source...
  18. MATĚJKOVÁ ©., GUT I., 2000. Polyphenols in nutrition as protective compounds. Remedia, 10: 272-281.
  19. MONTERO C., CRISTESCU S.M., JIMENEZ J.B., OREA J.M., HEKKERT S.T.L., HARREN F.J.M., URENA A.G., 2003. Trans-resveratrol and grape disease resistance. A dynamical study by high-resolution laser-based techniques. Plant Physiol., 131: 129-138. Go to original source... Go to PubMed...
  20. MORALES M., ALCÁNTARA J., BARCELÓ A.R., 1997. Oxidation of trans-resveratrol by a hypodermal peroxidase isoenzyme from Gamay rouge grape (Vitis vinifera) berries. Am. J. Enol. Vitic., 48: 33-39. Go to original source...
  21. SCHMANDKE H., 2002. Resveratrol and piceid in grapes and soybeans and products made from them. ErnährungsUmschau, 49: 349-352.
  22. ©MIDRKAL J., FILIP V., MELZOCH K., HANZLÍKOVÁ I., BUCKIOVÁ D., KRI©A B., 2001. Resveratrol. Chem. Listy, 95: 602-609.
  23. UHLIG B.A., CLINGELEFFER P.R., 1998. Ripening characteristics of the fruit from Vitis vinifera L. drying cultivars sultana and Merbein seedless under furrow irrigation. Amer. J. Enol. Vitic., 49: 375-382. Go to original source...
  24. UHLIG B.A., 1998. Effects of solar radiation on grape (Vitis vinifera L.) composition and dried fruit colour. J. Hort. Sci. & Biotechnol., 73: 111-123. Go to original source...
  25. WANG H., CAO G., PRIOR R.L., 1996. Total antioxidant capacity of fruits. J. Agric. Food Chem., 44: 701-705. Go to original source...
  26. ZOECKLEIN B.W., WOLF T.K., DUNCAN S.E., MARCY J.E., JASINKI Y., 1998. Effect of fruit zone removal on total glycoconjugates and conjugate fraction concentration of Riesling and Chardonnay (Vitis vinifera L.) grapes. Amer. J. Enol. Vitic., 49: 259-265. Go to original source...

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.