Hort. Sci. (Prague), 2016, 43(3):142-148 | DOI: 10.17221/151/2015-HORTSCI

Effects of garlic genotype on cloves formation under in vitro conditionsOriginal Paper

H. Fišerová, T. Vyhnánek, Z. Staňková, V. Kozák, M. Klemš, L. Havel
Department of Plant Biology, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic

Prior to the transfer of multiplicated garlic plants from in vitro to ex vitro conditions it is necessary to induce the formation of bulbs and to verify the identity of propagated young individuals with their maternal plant. This study deals with effects of exogenous compounds (i.e. paclobutrazol, abscisic acid and ethylene) on formation of bulbs of four garlic cultivars (Lan, Lukan, Emilie, Japo) and on production of ethylene and carbon dioxide (CO2). In the softneck garlic cv. Emilie, the exogenous application of paclobutrazol increased numbers of daughter cloves and production of both ethylene and CO2. In the softneck cv. Lukan the formation of cloves was higher after the application of ABA than after the application of paclobutrazol (PP 333). An exogenous application of ethylene inhibited the formation of bulbs. Analyses of DNA polymorphism, performed by means of microsatellite markers, verified the identity of bulbs produced under in vitro conditions with their mother plants. The hardneck cv. Lan differed form softneck cvs Lukan, Emilie and Japo.

Keywords: paclobutrazol; abscisic acid; 2-chlorethylphosphonic acid; ethylene; microsatellites

Published: September 30, 2016  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Fišerová H, Vyhnánek T, Staňková Z, Kozák V, Klemš M, Havel L. Effects of garlic genotype on cloves formation under in vitro conditions. Hort. Sci. (Prague). 2016;43(3):142-148. doi: 10.17221/151/2015-HORTSCI.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Biran I., Leshem B., Gur I., Halevy A.H. (1974): Further studies on relationship between growth regulators and tuberization of dahlias. Physiologia Plantarum, 31: 23-28. Go to original source...
    2. Burg S.P., Burg E.A. (1968): Ethylene formation in pea seedlings. Its relation to the inhibition of bud growth caused by indole-3-acetic acid. Plant Physiology, 43: 1069-1074. Go to original source... Go to PubMed...
    3. Cvikrová M., Sukhova L.S., Eder J., Korableva N.P. (1994): Possible involvement of abscisic acid, ethylene and phenolic acids in potato tuber dormancy. Plant Physiology and Biochemistry, 32: 685-691.
    4. Davis T.D., Haissig B.E., Sankhla N. (1991): Adventitious root formation in cuttings. Advances in Plant Sciences Series. Biologia Plantarum, 33: 114. Go to original source...
    5. De Mattia F., Imazio S., Grassi F., Lovicu G., Tardaguila J., Failla O., Maitt Ch., Scienza A., Labra M. (2007): Genetic characterization of Sardinia grapevine cultivars by SSR markers analysis. Journal International des Sciences de la Vigne et du Vin, 41: 175-184. Go to original source...
    6. England D.J.F. (1978): PP 333 - A new grass retardant. In: I. H. Rorison and R. Hunt (eds): Amenity Grassland: An Ecological Perspective. Chichester, Wiley.
    7. Fišerová H., Kula E., Klemš M., Reinöhl V. (2001): Phytohormones as indicators of the degree of damage in birch. Biológia, 56: 405-409.
    8. Fišerová H., Klemš M., Čepl J., Macháčková I.,Procházka S. (2004): Dynamics of ethylene and abscisic acid levels during the growth of potato tubers. Acta Physiologie Plantarum, 26: 18.
    9. Fišerová H., Mikušová Z., Klemš M. (2008): Estimation of ethylene production and 1-aminocyclopropane-1-carboxylic acid content in plants by means of gas chromatography. Plant, Soil and Environment, 54: 55-60. Go to original source...
    10. Fišerová H., Prokeš J., Helánová A., Hartmann J. (2010): Změny kvality sladu v průběhu posklizňového dozrávání ječmene. Kvasný průmysl, 58: 93-99. Go to original source...
    11. Hampl V., Pavlíček A., Flegr J. (2001): Construction and bootstrap analysis of DNA fingerpriting-based phylogenetic trees with a freeware program FreeTree: Application to Trichomonad parasites. International Journal of Systematic and Evolutionary Microbiology, 51: 731-735. Go to original source... Go to PubMed...
    12. Haque M.S., Wada T., Hattori K. (2000): Garlic roots for micropropagation through in vitro bulblet formation. Proceedings of the XXV. International Horticultural Congress, Pt 10: Application of Biotechnology and Molecular Biology and Breeding In Vitro Culture Book Series. Acta Horticulturae (ISHS), 520: 45-52. Go to original source...
    13. Havel L., Novák F.J. (1988): Regulation of somatic embryogenesis and organogenesis in Allium-carinatum L. Journal of Plant Physiology, 132: 373-377. Go to original source...
    14. Hradilík J., Fišerová H. (1986): Interakce mezi giberelinem (GA3) a paclobutrazolem (P 333) u salátu, hrachu a lnu. Acta Universitatis Agriculturae at Silviculturae (Brno), XXXV: 5-11.
    15. Hradilík J., Fišerová H. (1987): Vliv kyseliny giberelové (GA3) a paclobutrazolu (PP 333) na morfologii bramboříku. Acta Universitatis Agriculturae at Silviculturae (Brno), XXXV: 23-26.
    16. Jaccard P. (1908): Nouvelles recherches sur la distribution florale. Bulletin del la Societé Vaudoise des Scientes Naturelles, 44: 223-270.
    17. Králík J., Šebánek J. (1989): On the stimulating effects of paclobutrazol on the rhizogenezis of Ligustrum vulgare L. cuttings. Acta Universitatis Agriculturae at Silviculturae (Brno), 37: 51-54.
    18. Liu L.S., Li R., Zhao Y., Wen C.L., Ren S., Guo Y.D. (2011): High efficiency regeneration and genetic stability analysis of somatic clones of Gynura bicolor DC. African Journal of Biotechnology, 10: 10380-10386. Go to original source...
    19. Ma K.H., Kwag J.G., Zhao W., Dixit A., Lee G.A., Kim H.H., Chung I.M., Kim N.S., Lee J.S., Ji J.J., Kim T.S., Park Y.J. (2009): Isolation and characteristics of eight novel polymorphic microsatellite loci from the genome of garlic (Allium sativum L.). Scientia Horticulturae, 122: 355-361. Go to original source...
    20. Macháčková I., Ullmann J. (1987): Ethylene - a natural regulator of plant growth and development. Biologické listy, 52: 282-306.
    21. Murashige T., Skoog F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15: 473-97. Go to original source...
    22. Nagakubo T., Nagasawa A., Nagakubo T., Nagasawa A., Ohkawa H. (1993): Micropropagation of garlic through in vitro bulblet formation. Plant Cell Tissue and Organ Culture, 32: 175-183. Go to original source...
    23. Nambara E., Okamoto M., Tatematsu K., Yano R., Seo M., Kamiya Y. (2010): Abscisic acid and the control of seed dormancy and germination. Seed Science Research, 20: 55-67. Go to original source...
    24. Novák F.J., Havel L. (1981): Shoot production from in vitro cultured flower heads of Allium porrum L. Biologia Plantarum, 23: 266-269. Go to original source...
    25. Ovesná J., Leišová-Svobodová L., Kučera L. (2014): Microsatellite analysis indicates the specific genetic basis of Czech bolting garlic. Czech Journal of Genetics and Plant Breeding, 50: 226-234. Go to original source...
    26. Page R.D.M. (1996): Treeview: An application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences, 12: 357-358. Go to original source... Go to PubMed...
    27. Phulwaria M., Rai M.K., Shekhawat N.S. (2013): An improved micropropagation of Arnebia hispidissima (Lehm.) DC. and assessment of genetic fidelity of micropropagated plants using DNA-based molecular markers. Applied Biochemistry and Biotechnology, 170: 1163-1173. Go to original source... Go to PubMed...
    28. Prokeš J., Fišerová H., Helánová A., Hartmann J. (2006): Význam oxidu uhličitého a ethylenu v procesu sladování. Kvasný průmysl, 52: 349-352. Go to original source...
    29. Ridge I., Amarashige I. (1984): Ethylene and growth-control in the fringend waterlily (Nymphoides-peltata)-stimulation of cell-division and interaction with budyant pension in petioles. Plant Growth Regulation, 2: 235-249. Go to original source...
    30. Russell J., Fuller J., Young G., Thomas B., Taramino G., Macaulay M., Waugh R., Powel W. (1997): Discriminating between barley genotypes using microsatellite markers. Genome, 40: 442-450. Go to original source... Go to PubMed...
    31. Sohn E.Y., Kim Y.H., Kim J.T., Jang S.W., Lee I.J. (2011): Changes in endogenous abscisic acid, jasmonic acid and sucrose content during the bulb development in the coldtype cultivar of garlic (Allium sativum L.) of Korea. Korean Journal of Horticultural Science & Technology, 29: 1-9.
    32. Šebánek J., Klíčová Š., Králík J., Psota V., Vitková H., Kudová D., Reinöhl V. (1991): The effect of paclobutrazol on the level of endogenous IAA in relation to the rooting of cuttings and abscission of petioles. Biochemie und Physiologie der Pflanzen, 187: 89-94 Go to original source...
    33. Vyhnánek T., Nevrtalová E., Slezáková K. (2009): Detection of the genetic variability of triticale using wheat and rye SSR markers. Cereal Research Communications, 37: 23-29. Go to original source...
    34. Yang S.F. (1980): Regulation of ethylene biosynthesis. Horticulturae Science. 15: 238-243. Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content