Horticultural Science, 2009 (vol. 36), issue 4

Index of Volume 36Index

editors

Hort. Sci. (Prague), 2009, 36(4):I-II | DOI: 10.17221/2909-HORTSCI  

DNA polymorphism in genetic resources of red pepper using microsatellite markers

P. Hanáček, T. Vyhnánek, M. Rohrer, J. Cieslarová, H. Stavělíková

Hort. Sci. (Prague), 2009, 36(4):127-132 | DOI: 10.17221/7/2009-HORTSCI  

Genetic variability among 41 accessions of red pepper (Capsicum annuum L.) was assessed using eight microsatellite markers. Three of the microsatellite markers (Hpms 1-1, Hpms 1-168, and Hpms 1-274) had uniform spectra in all the analyzed plants. Two to eight alleles were detected for the remaining loci. In total, 28 alleles were detected, i.e. 3.5 alleles per one microsatellite locus on average. The highest number of different alleles was detected with Hpms 1-5 (8 alleles) and Hpms 2-21 primers (7 alleles). Molecular data were complemented with morphological measurements according to the descriptor list for the genus Capsicum....

Application of synchrotron radiation X-ray fluorescence to investigate the distribution of mineral elements in different organs of greenhouse spinach

S. Z. Xin, Y. J. Song, C. Lv, Y. K. Rui, F. S. Zhang, W. Xu, D. Wu, S. Wu, J. Zhong, D. L. Chen, Q. Chen, F. T. Peng

Hort. Sci. (Prague), 2009, 36(4):133-139 | DOI: 10.17221/18/2009-HORTSCI  

Consumption of vegetables is one of the most important ways of providing the body with mineral elements. However, it is not clear how mineral elements are distributed in different organs of vegetables, especially vegetables grown in greenhouses. The distribution of mineral elements in the root and leaves of greenhouse spinach was determined using synchrotron radiation XRF, the results indicated that the amount of various elements in different parts of the leaves, roots and stems were inequable. Generally, the content of the elements in the root base were slightly higher than in the other parts. The amounts in the root apices were much lower than those...

Influence of growth regulators and explants on shoot regeneration in carnation

J. K. Kanwar, S. Kumar

Hort. Sci. (Prague), 2009, 36(4):140-146 | DOI: 10.17221/1/2009-HORTSCI  

The influence of growth regulators, explants and their interactions on in vitro shoot bud formation from callus was studied in Dianthus caryophyllus L. The leaf and internode explants were cultured on Murashige and Skoog (MS) medium containing different concentrations of growth regulators. The highest callus induction was observed with 2 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D) and 1 mg/l benzyl adenine (BA). Out of twenty seven shoot regeneration media tested, only 2 mg/l thidiazuron (TDZ) and zeatin alone or in combination with naphthalene acetic acid (NAA) and/or indole acetic acid (IAA) could differentiate calli. The highest average...

Occurrence and correction of chlorosis in young petunia plants

F. Šrámek, M. Dubský

Hort. Sci. (Prague), 2009, 36(4):147-153 | DOI: 10.17221/6/2009-HORTSCI  

: A glasshouse pot experiment tested the effects of 14 different combinations of substrate type, pH and nutrient treatments on the occurrence and severity of leaf chlorosis in a susceptible variety of petunia. Plants grown at optimal pH level (4.7) in peat substrate with low limestone dose were symptom-free even without added micronutrients. Severe chlorosis occurred in plants grown at high pH in peat substrate with high limestone (pH 6.7) and in peat-bark-compost (pH 6.2); it was associated with decreased Fe and Mn content in leaves. Regular application of nutrient solution with low concentration of Fe, Mn, and other micronutrients as EDTA chelates...

Testing of Koch method applied for evaluation of ornamental trees in the Czech Republic

P. Bulíř

Hort. Sci. (Prague), 2009, 36(4):154-161 | DOI: 10.17221/60/2008-HORTSCI  

Koch method of ornamental trees evaluation is based on interest paid on costs invested in tree-growing up to their maturity stage minus deductions for age, defects and damage. The aim of this paper is to apply the above mentioned method in the economic conditions of the Czech Republic. The paper presents essential economic data generated in accordance with the technological model of tree growing at a permanent site. We also describe the mechanism of the so-called base tree price calculation as a basis for the subtraction of deductions derived from its current condition. The calculated base tree prices are illustratively compared to prices based on...

Cultivation of Cypripedium calceolus L. ex vitro seedlings in outdoor conditions: Short communication

J. Obdržálek

Hort. Sci. (Prague), 2009, 36(4):162-170 | DOI: 10.17221/4/2009-HORTSCI  

The paper presents first positive results of the experiment with three-year outdoor cultivation of Cypripedium calceolus L. ex vitro seedlings in the Czech Republic. They were propagated in vitro from mature seeds of Carpathian provenance in a Prague private laboratory. In April 2006 after three months cool refrigerating at 4°C the seedlings were prepared for planting. The rhizomes with 4 to 12 roots and visible dormant buds were used. They were planted in two types of substrates: mixture AN on the basis of liadrain (burned clay pebbles) and mixture BN on the basis of granodiorite. Both mixtures were amended with perlite,...