Hort. Sci. (Prague), 2020, 47(1):69-79 | DOI: 10.17221/83/2018-HORTSCI

Molecular characterisation of apple accessions with respect to aminocyclopropane-1-carboxylic acid synthase gene (ACS1) polymorphismOriginal Paper

Khethani Give Mhelembe*,1,2, Johan Hendrik Kriel1, Cecilia Bester1, Rouvay Roodt-Wilding2, Esmé Denise Louw3, Kenneth Richard Tobutt1
1 Agricultural Research Council Infruitec-Nietvoorbij, Stellenbosch, South Africa
2 Department of Genetics, Stellenbosch University, Matieland, South Africa
3 Department of Horticultural Science, Stellenbosch University, Matieland, South Africa

The ARC apple gene bank collection was genotyped for the fruit expressed gene ACS1, in which a short-interspersed element (SINE) in the promoter is known, when homozygous, to correlate with the delayed ethylene production. Primers were designed amplifying products less than 500 bp and 224 cultivars of domestic apple were analysed, 169 not previously genotyped. Of these, 82 were aa (homozygous for the high ethylene allele at 202 bp), 73 were ab and 14 bb (homozygous for the low ethylene allele, with the SINE, at 339 bp). The difference between the allele sizes, 137 bp, observed in the current study is consistent with the indel of 138 bp originally described, but differs considerably from the indel of 166 bp reported in literature. In addition, 21 accessions of other Malus species were analysed. Only one, M. 'Golden Hornet', had the b allele, which suggests it may have been introgressed from M. pumila.

Keywords: apple; fruit quality; genotyping; storability; Malus sp.; marker-assisted selection

Published: March 31, 2020  Show citation

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Mhelembe KG, Kriel JH, Bester C, Roodt-Wilding R, Louw ED, Tobutt KR. Molecular characterisation of apple accessions with respect to aminocyclopropane-1-carboxylic acid synthase gene (ACS1) polymorphism. Hort. Sci. (Prague). 2020;47(1):69-79. doi: 10.17221/83/2018-HORTSCI.
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