Hort. Sci. (Prague), 2018, 45(3):125-130 | DOI: 10.17221/212/2016-HORTSCI

Does dikegulac affect in vitro shoot proliferation and hyperhydricity incidence in olive explants?Original Paper

Chrysovalantou Antonopoulou*,1, Kortessa Dimassi1, Ioannis Therios1, Christos Chatzissavvidis2
1 Laboratory of Pomology, School of Agriculture, Aristotle University of Thessaloniki, Thessaloniki, Greece
2 Department of Agricultural Development, School of Agricultural Sciences and Forestry, Democritus University of Thrace, Orestiada, Greece

Dikegulac was tested as a lateral shoot-inducing agent on micropropagated olive (Olea europaea 'Chondrolia Chalkidikis') shoots. Rugini olive medium was supplemented with dikegulac at 0, 16.9, 33.8, 66.7, 100.5 or 133.4 μΜ. Dikegulac was not phytotoxic and the explants treated with 100.5 μΜ had higher number, length, and weight of new shoots. Hyperhydricity (or vitrification) symptoms were diminished by increasing dikegulac concentration in the medium dose (66.7-133.4 μΜ). Also, dikegulac stimulated the production of large amounts of callus at the base of olive explants.

Keywords: apical dominance; growth retardant; micropropagation; Olea europaea, vitrification

Published: September 30, 2018  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Antonopoulou C, Dimassi K, Therios I, Chatzissavvidis C. Does dikegulac affect in vitro shoot proliferation and hyperhydricity incidence in olive explants? Hort. Sci. (Prague). 2018;45(3):125-130. doi: 10.17221/212/2016-HORTSCI.
Download citation

References

  1. Antonopoulou C. (2009): The effect of various parameters on hyperhydricity, shoot production and rooting of olive (Olea europaea L.) in vitro. [PhD Thesis.] Aristotle University, Thessaloniki, Greece. (In Greek, with English abstract).
  2. Antonopoulou C., Dimassi K., Chatzissavvidis C., Therios I., Papadakis I. (2006): Effect of BA and GA3 on the micropropagation and vitrification of olive (Olea europaea L.) explants (cv. Chondrolia Chalkidikis). In: Olivebioteq Proceedings of the 2nd International Seminar on Biotechnology and Quality of Olive Tree Products Around the Mediterranean Basin, vol. 1, Sicily, Italy: 477-480.
  3. Antonopoulou C., Dimassi K., Chatzissavvidis C., Papadakis I., Therios I. (2012): The effect of explant type and nutrient medium on the in vitro proliferation of olive (Olea europaea L. 'Chondrolia Chalkidikis). Acta Horticulturae (ISHS), 949: 185-189. Go to original source...
  4. Bi G., Gu M. (2015): Effects of pruning and plant growth regulators on branching and growth of azalea. Acta Horticulturae (ISHS), 1104: 109-113. Go to original source...
  5. Bocion P.F., de Silva W.H. (1977): Some effects of dikegulac on the physiology of whole plants and tissues: Interactions with plant hormones. In: Pilet P.E. (eds.): Plant Growth Regulation. Proceedings in Life Sciences, Springer, Berlin, Heidelberg: 189-198. Go to original source...
  6. Burns J.A., Smith L.A., Ward D.C. (2002): Novel multiple shoot proliferation and regeneration system for plants. Patent Cooperation Treaty International Application.
  7. Choudhury S., Gupta K. (1999): Effect of dikegulac on biomass and alkaloid production in Catharanthus roseus (L.) G. Don under in vitro condition. Indian Journal of Experimental Biology, 37: 594-598.
  8. Cline M.G. (1996): Exogenous auxin effects on lateral bud outgrowth in decapitated shoots. Annals of Botany, 78: 255-266. Go to original source...
  9. Cline M.G. (1997): Concepts and terminology of apical dominance. American Journal of Botany, 84: 1064-1069. Go to original source...
  10. Cochran D.R., Fulcher A. (2013): Type and rate of plant growth regulator influence vegetative, floral growth, and quality of Little Lime™ Hydrangea. HortTechnology, 23: 306-311. Go to original source...
  11. Dimassi K. (1999): Micropropagation studies of the cv. Kalamon olives (Olea europaea sativa L.). Acta Horticulturae (ISHS), 474: 83-86. Go to original source...
  12. Dimassi-Theriou K. (1994): In vitro propagation of cv. Kalamon olives (Olea europaea sativa L.). Advances in Horticultural Science, 8: 185-189.
  13. Ebrahim M.K.H. (2004): Comparison, determination and optimizing the conditions required for rhizome and shoot formation, and flowering of in vitro cultured calla explants. Scientia Horticulturae, 101: 305-313. Go to original source...
  14. Grigoriadou K., Vasilakakis M., Eleftheriou E.P. (2002): In vitro propagation of the Greek olive cultivar Chondrolia Chalkidikis. Plant Cell Tissue & Organ Culture, 71: 47-54. Go to original source...
  15. Grigoriadou K., Eleftheriou E.P., Vasilakakis M. (2007): Hidden hyperhydricity may be responsible for abnormal development and acclimatization problems of micropropagated olive plantlets: An anatomical leaf study. Acta Horticulturae (ISHS), 748: 103-106. Go to original source...
  16. Grossman M., Freeborn J., Scoggins H., Latimer J. (2013): Timing of benzyladenine and dikegulac sodium application affects branching of liners and finished herbaceous perennial plants. HortScience, 48: 875-882. Go to original source...
  17. Jacyna T., Starbuck C.J., Ellersiek M.R. (1994): Increasing branching of landscape pear trees with promalin and dikegulac-sodium. Journal of Environmental Horticulture 12: 90-92. Go to original source...
  18. Kataeva N.V., Alexandrova I.G., Butenko R.G., Dragavtcena E.V. (1991): Effect of applied and internal hormones on vitrification and apical necrosis of different plants cultured in vitro. Plant Cell Tissue & Organ Culture, 27: 149-154. Go to original source...
  19. Latimer J., Freeborn J. (2010): Branching enhancers, Configure (6-BA) and Augeo (Dikegulac sodium), affect branching of herbaceous perennials. In: Proceedings of the 38th Annual Meeting of the Plant Growth Regulation Society of America, USA: 148-152.
  20. Litwinczuk W., Prokop A. (2010): The usefulness of dikegulac in propagation of highbush blueberry (Vaccinium corymbosum L.) 'Herbert'. Journal of Fruit & Ornamental Plant Research, 18: 85-92.
  21. Martin G., Geetha S.P., Raja S.S., Raghu A.V., Balachandran I., Ravindran P.N. (2006): An efficient micropropagation system for Celastrus paniculatus Wild.: A vulnerable medicinal plant. Journal of Forest Research, 11: 461-465. Go to original source...
  22. Mendoza-De Gyves E., Rosana M.F., Ruiu F., Rugini E. (2008): Stimulation of node and lateral shoot formation in micropropagation of olive (Olea europaea L.) by using dikegulac. Plant Cell Tissue & Organ Culture, 92: 233-238. Go to original source...
  23. Nir G., Shulamn Y., Lavee S. (1983): Effects of dikegulac on the vegetative development of grapevine (Vitis vinifera) and olive (Olea europaea) shoots. Scientia Horticulturae, 21: 147-153. Go to original source...
  24. Norcini J.G., Aldrich J.H., McDowell J.M. (1994): Flowering response of Bougainvillea cultivars to dikegulac. HortScience, 29: 282-284. Go to original source...
  25. Paques M., Boxus P. (1987): Vitrification: A review of literature. Acta Horticulturae (ISHS), 212: 155-166. Go to original source...
  26. Peixe A., Raposo A., Lourenco R., Cardoso H., Macedo E. (2007): Coconut water and BAP successfully replaced zeatin in olive (Olea europaea L.) micropropagation. Scientia Horticulturae, 113: 1-7. Go to original source...
  27. Pozo L., Redondo A., Hartmond U., Kender W.J., Burns J.K. (2004): Dikegulac promotes abscission in citrus. HortScience, 39: 1655-1658. Go to original source...
  28. Puglisi S.E. (2002): Use of plant growth regulators to enhance branching of Clematis spp. M.Sc. Thesis, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA.
  29. Rugini E.P. (1984): In vitro propagation of some olive (Olea europaea sativa L.) cultivars with different root-ability, and medium development using analytical data from developing shoots and embryos. Scientia Horticulturae, 24: 123-134. Go to original source...
  30. Rugini E. (1990): In vitro culture of olive: an overview of the present scientific status. Acta Horticulturae (ISHS), 286: 93-96. Go to original source...
  31. Rugini E., Panelli G. (1993): Preliminary results on increasing fruit set in olive (Olea europaea L.) by chemical and mechanical treatments. Acta Horticulturae (ISHS), 329: 209-220. Go to original source...
  32. Rugini E., Guttierez-Pesce P., Sampinato P.L. (1999): New perspective for biotechnologies in olive breeding: morphogenesis, in vitro selection and gene transformation. Acta Horticulturae (ISHS), 474: 107-110. Go to original source...
  33. Sachs R.M., Hield H., Debie J. (1975): Dikegulac, a promising new foliar-applied growth regulator for woody species. HortScience, 10: 367-369. Go to original source...
  34. Sansberro P., Mroginski L., Bottini R. (2006): Stimulation of lateral branch formation on Ilex paraguariensis (Aquifoliaceae) seedlings. Australian Journal of Experimental Agriculture, 46: 707-710. Go to original source...
  35. Sarropoulou V., Dimassi-Theriou K., Therios I. (2014): Dikegulac-sodium effect on micropropagation and biochemical parameters in the cherry rootstocks CAB-6P and Gisela 6. Turkish Journal of Agricultural and Natural Science, 2: 1713-1726. Go to original source...
  36. Sghir S., Chatelet P., Ouazzani N., Dosba F., Belkoura I. (2005): Micropropagation of eight Moroccan and French olive cultivars. HortScience, 40: 193-196. Go to original source...
  37. Sun Y., Bi G., Niu G., Perez C. (2015): Foliar application of dikegulac sodium increases branching of 'Merritt's Supreme' bigleaf hydrangea. HortTechnology, 3: 306-312. Go to original source...
  38. Thetford M., Berry J.B. (2000): Response of five woody landscape plants to Primo and pruning. Journal of Environmental Horticulture, 18: 128-132. Go to original source...
  39. Whiting P.A. (2007): Commercial production of Christia subcordata Moench by establishing cultural practices and by applying plant growth regulators. [MSc Thesis.] University of Georgia, Athens.
  40. Wright D.C., Moran J.T. (1998): Effect of Atrinal (Dikegulac) on the growth of plane tree, red maple and Norway maple in New York. Journal of Arboriculture, 14: 121-124. Go to original source...
  41. Zuccherelli G., Zuccherelli S. (2002): In vitro propagation of fifty olive cultivars. Acta Horticulturae (ISHS), 586: 931-934. Go to original source...

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.