Hort. Sci. (Prague), 2016, 43(2):100-104 | DOI: 10.17221/326/2014-HORTSCI

Molecular cloning and characterization of allele d ‒ a newly identified allele of the 1-aminocyclopropane-1-carboxylate oxidase 1 (ACO1) gene in apple - Short CommunicationShort Communication

S. Marić
Department of Pomology and Fruit Breeding, Fruit Research Institute, Čačak, Republic of Serbia

Apple is a climacteric fruit of economic importance and high nutritional value. Better understanding of the apple ripening process is needed to improve fruit quality and extend apple shelf life. This paper presents the results of molecular cloning and characterization of a newly identified allele of the ripening-specific ACO1 gene from Malus floribunda 821. A polymorphism of the ACO1 gene was detected using the polymerase chain reaction and BamH1 and RsaI enzymatic digestion. Whilst cloning and sequencing data confirmed the identity of allele d, it also revealed high sequence conservation (97.3 to 98.9%). In addition, a comparison of sequence data revealed some differences within the coding regions of the ACO1 alleles identified so far.

Keywords: Malus floribunda 821; polymorphism of ACO1 gene; ripening; ethylene

Published: June 30, 2016  Show citation

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Marić S. Molecular cloning and characterization of allele d ‒ a newly identified allele of the 1-aminocyclopropane-1-carboxylate oxidase 1 (ACO1) gene in apple - Short Communication. Hort. Sci. (Prague). 2016;43(2):100-104. doi: 10.17221/326/2014-HORTSCI.
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