Hort. Sci. (Prague), 2016, 43(2):84-91 | DOI: 10.17221/351/2014-HORTSCI

Effect of warm and cold stratification, and ethanol treatment on germination of Corylopsis seedsOriginal Paper

J.H. Kim, A.K. Lee, J.K. Suh
Department of Environmental Horticulture, College of Bio-Resources Science, Dankook University, Cheonan, Korea

Seed germination experiments with Corylopsis coreana, C. sinensis var. calvescens, and Corylopsis gotoana were conducted. Corylopsis coreana seeds were treated at 5o, 7.5o, 10o, 12.5o and 20oC to study the upper temperature limit to break dormancy. Seeds of C. sinensis var. calvescens were treated 1 mo of warm stratification (WS) at 10, 15, 20, and 25oC followed by CS at 5oC for 0, 1, 2, and 3 mo to understand the requirement of WS and CS to effectively break dormancy. Germination of Corylopsis seeds at 15o-20oC without CS suggests that dormancy is considered shallow and the upper temperature limit to break is 12.5oC. Immersing seeds in water or ethanol induced seed germination which may result from leaching of inhibitors from the seed. Observation of seed coats following treatment with water and ethanol by means of low temperature scanning electron microscopy (LT-SEM) revealed elongated and rectangular/hexagonal shaped cells in C. coreana and irregular jigsaw puzzle-piece shapes in C. gotoana. In conclusion, dormancy in Corylopsis is considered shallow and the optimum CS is suggested to be 7.5oC, while optimum WS is ranging from12.5o to 20oC.

Keywords: Corylopsis coreana, Corylopsis gotoana, Corylopsis sinensis var. calvescens, optimum temperature, scanning electron microscopy, seed dormancy

Published: June 30, 2016  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Kim JH, Lee AK, Suh JK. Effect of warm and cold stratification, and ethanol treatment on germination of Corylopsis seeds. Hort. Sci. (Prague). 2016;43(2):84-91. doi: 10.17221/351/2014-HORTSCI.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Ali-Rachedi S., Bouinot D., Wagner M.-H, Bonnet M., Scotta B., Garppins P., Jullien M. (2004): Changes in endogenous abscisic acid levels during dormancy release and maintenance of mature seeds: studies with the Cape Verde Islands ecotype, the dormant model of Arabidopsis thaliana. Planta, 219: 479-488. Go to original source... Go to PubMed...
    2. Barbary O.M., Al-sohaimy S.A., El-Saadani M.A., Zeitoun A.M.A. (2009): Extraction, composition and physicochemical properties of flaxseed mucilage. Journal of The Advanced Agriculture Researches, 14: 605-622.
    3. Baskin J.M., Baskin C.C (2004): A classification system for seed dormancy. Seed Science Research, 14: 1-16. Go to original source...
    4. Baskin C.C., Zackrisson O., Baskin J.M. (2002): Role of warm stratification in promoting germination of Empetrum hermaphroditum (Enoetraceae), a critical species with a stony endocarp. American Journal of Botany, 89: 486-493. Go to original source... Go to PubMed...
    5. Batlla D., Benech-Arnold R.I. (2003): A quantitative analysis of dormancy loss dynamics in Polygonium aviculare L. seeds: development of a thermal time model based on changes in seed population thermal parameters. Seed Science Research, 13: 55-68. Go to original source...
    6. Cavanagh A.K. (1980): Some aspects of the history of seed coat treatments applied to acacias. International Group for the Study of Mimosoideae. Bulletin, 8: 31-36.
    7. Dirr M. (1990): Manual of woody landscape plants: Their identification, ornamental characteristics, culture, propagation and uses. Champaign, Stipes Publishing Co. Haughn.
    8. Chaudhury A. (2005): Genetic analysis of seed coat development in Arabidopsis. Trends in Plant Science, 10: 472-477. Go to original source... Go to PubMed...
    9. Hilhost H.W.M. (2008): Definition and hypothesis of seed dormancy. In: Bradford K., Nonogaki H. (eds): Seed Development, Dormancy and Germination. Oxford, Blackwell Publishing: 50-71. Go to original source...
    10. Horimoto T., Koshioka M., Kubota S., Mander L. N., Hirai N., Ishida N., Suh J. K., Lee A. K., Roh M. S. (2011): Effect of warm and cold stratification on 1H-NMR profiles, endogenous gibberellins and abscisic acid in Styrax japonicus seeds. Horticulture, Environment, and Biotechnology, 52: 233-239. Go to original source...
    11. Kwon H.J. (1995): Ecological characteristics, propagation method, and new cultivar of Korean Styrax japonicus Sieb. et Zucc. for landscape tree uses. [Ph.D. Thesis.] Sung Kyun Kwan Univeristy, Suwon, Korea (in Korean).
    12. Liu S.X., White E. (2012): Extraction and characterization of proanthocyanidins from grape seeds. The Open Food Science Journal, 6: 5-11. Go to original source...
    13. Moon H.K., Noh E.W., Ha Y.M., Shim K.K. (2002): Micropropagation of juvenile and mature tree of Corylopsis coreana by axillary bud culture. Korean Journal Plant Biotechnology, 29: 117-121. Go to original source...
    14. Pipinis E., Milios E., Aslanidou M., Mavrokordopoulou O., Smiris P. (2009): The effect of stratification on seed germination of Jasminus fruticans L. (Oleaceae): A contribution to a better insight on the species germination ecology. International Journal of Botany, 5: 181-185. Go to original source...
    15. Rehman S., Park I-H. (2000): Effect of scarification, GA and chilling on the germination of goldenrain-tree (Koelreuteria panicularata Laxm.) seeds. Scientia Horticulturae, 85: 319-324. Go to original source...
    16. Roh M.S., Bentz J-A., Wang P., Li E., Koshioka M. (2004): Maturity and temperature stratification affect the germination of Styrax japonicus seeds. The Journal of Horticultural Science and Biotechnology, 79: 645-651. Go to original source...
    17. Roh M.S., Lee A.K., Suh J.K., Bordelon C.M (2008): Interspecific variations in seed germination of Corylopsis. Scientia Horticulturae, 118: 347-350. Go to original source...
    18. Siddiqui Z.S., Khan M.A. (2010): The role of seed coat phenolics on water uptake and early protein synthesis during germination of dimorphic seeds of Halopyrum mucronatum (L.) Spath. Pakistan Journal of Botany, 42: 227-238.
    19. Suzuki T., Matsuura T., Kawakami N., Noda K. (2000): Accumulation and leakage of abscisic acid during embryo development and seed dormancy in wheat. Plant Growth Regulation, 30: 253-260. Go to original source...
    20. Western T.L. (2012): The sticky tale of seed coat mucilages: production, genetics, and role in seed germination and dispersal. Seed Science Research, 22: 1-25. Go to original source...
    21. Taylorson R.B., Hendricks S.B. (1979): Overcoming dormancy in seeds with ethanol and other anesthetics. Planta, 145: 507-510. Go to original source... Go to PubMed...
    22. Wada S., Kennedy J. A., Reed B.M. (2011): Seed-coat anatomy and proanthocyanidins contribute the dormancy of Rubus seed. Scientia Horticulturae, 130: 762-768. Go to original source...
    23. Young J.A., Young C.G. (1992): Seeds of woody plants in North America. Portalnd, Dioscorides Press. Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content