Hort. Sci. (Prague), 2015, 42(1):13-21 | DOI: 10.17221/103/2014-HORTSCI

Identification of S-genotypes of sweet cherry cultivars from Central and Eastern EuropeOriginal Paper

A. Lisek¹, E. Rozpara¹, A. Głowacka¹, D. Kucharska², M. Zawadzka³

¹ Cultivar Evaluation Laboratory, Research Institute of Horticulture, Skierniewice, Poland

² Microscopy Laboratory, Research Institute of Horticulture, Skierniewice, Poland

³ Włocławek, Poland

Sweet cherry is a species that is characterized by self-incompatibility, which causes difficulties in obtaining high yields of sweet cherry fruit. Determination of the composition of the S-alleles of sweet cherry cultivars is useful both to growers producing the fruit and breeders when selecting cultivars for cross-fertilizations. In this work, S-alleles have been identified for 47 sweet cherry cultivars from Central and Eastern Europe, mostly from Ukraine and the Czech Republic, with S-genotypes of 43 cultivars identified for the first time. To identify the S-alleles, PCR-based methods were used. In each cultivar, two of the following eight S-alleles were identified: S₁, S₂, S₃, S₄, S₅, S₆, S₉ and S₁₃. In the cultivars from the Czech Republic, the most frequently occurring were the alleles S₃, S₁ and S₄. In the cultivars originating in Ukraine, the most frequently found were the alleles S₅ and S₉, which makes them differ considerably from the sweet cherry cultivars from other regions of Europe. The tested sweet cherry cultivars were assigned to 20 of the existing incompatibility groups.

Keywords: Prunus avium L.; self-incompatibility; S-RNase; incompatibility groups; allele frequency

Published: March 31, 2015  Show citation