Hort. Sci. (Prague), 2009, 36(1):31-37 | DOI: 10.17221/29/2008-HORTSCI
Polyploidization of Pelargonium × hortorum L. H. Bailey in greenhouse conditions
- 1 Faculty of Horticulture, Mendel University of Agriculture and Forestry in Brno, Lednice, Czech Republic
- 2 Silva Tarouca Research Institute for Landscape and Ornamental Gardening, Průhonice, Czech Republic
This study is aimed at induction of polyploidy in the black-leaved cultivar Pelargonium × hortorum L. H. Bailey Black Velvet Scarlet F1 to obtain basic breeding material for creating new black-leaved tetraploid cultivars. The cultivar Gizela F1 was chosen as a control for the experiment. Tetraploidy was induced in seedlings in the cotyledon stage using various concentrations (from 0.1 to 2.5%) of colchicine water solutions; the treatments were repeated daily for 2, 3, 5 or 7 successive days. The first experiment, done in 2005, was very successful; 17.4% of treated Black Velvet Scarlet F1 plants and 23.7% of treated Gizela F1 plants were tetraploid, and other ploidy levels were also found. However, two other replications from 2006 (involving only five best treatments from the first experiment) were much less successful in comparison with the first one.
Keywords: Pelargonium × hortorum ; zonal pelargonium; Black Velvet Scarlet F1; colchicine; induced polyploidy; flow cytometry
Published: March 31, 2009 Show citation
References
- ACQUAAH G., 2007. Principles of Plant Genetics and Breeding. Oxford, Blackwell Publishing: 218-220.
- CHODOVÁ D., MIKULKA J., JOKEŠ M., 2004. Changes in Kochia (Kochia scoparia /L./ Schrad.) ploidy in relation to resistance to acetolactate synthase inhibitors. In: Proceedings of the XVI. Slovak and Czech Plant Protection Conference. Acta Fytotechnica et Zootechnica, 7 - Special Issue. Nitra, Slovak Agricultural University: 104-106.
- DOLEŽEL J., 1997. Application of flow cytometry for the study of plant genomes. Journal of Applied Genetics, 38: 285-302.
- HASSENEIN A., DORION N., 2006. Determining morphological and physiological parameters for the selection of drought-tolerant geraniums (Pelargonium × hortorum L. H. Bailey). Journal of Horticultural Science and Biotechnology, 81: 707-714.
Go to original source...
- HOFMANN CH., 1992. Schöne Pelargonien (Geranien). Stuttgart, Eugen Ulmer: 57.
- KOUTOULIS A., ROY A.T., PRICE A., SHERIFF L., LEGGETT G., 2005. DNA ploidy level of colchicine-treated hops (Humulus lupulus L.). Scientia Horticulturae, 105: 263-268.
Go to original source...
- LAGIBO A.D., KOBZA F., SUCHÁNKOVÁ P., 2005. Polyploidy effects on frost tolerance and winter survival of garden pansy genotypes. Horticultural Science (Prague), 32: 138-146.
Go to original source...
- LI M., 2005. Anatomische, cytologische und histologische Untersuchungen zur somatischen Variation in verschiedenen Teilklonen von Pelargonium znale Kleiner Liebling. [Dissertation.] Berlin, Humboldt University: 44.
- PLAVCOVÁ O., 2007. Leaf zonation of Pruhonice's brown leaved F1 varieties of Pelargonium zonale hort. In: Strom a květina - součást života. Sborník z vědecké konference, 4.-5. 9. 2007, Průhonice. Průhonice, VÚKOZ, v.v.i.: 257-259.
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