Hort. Sci. (Prague), 2008, 35(2):90-94 | DOI: 10.17221/643-HORTSCI

Growth regulator effect on in vitro regeneration of rhododendron cultivars

H. Vejsadová
Department of Biodiversity, Silva Tarouca Institute for Landscape and Ornamental Gardening, Průhonice, Czech Republic

In Rhododendron L. cv. Azuro, Bohumil Kavka, Catharine van Toll, Grandiflorum, Mars, Nova Zembla, Ortrud, Ovation, Prof. Scholz, Purple Splendour, Rebe and Van Werden Poelman, the effect of growth regulators on organogenesis induction of shoot-tip meristems was tested. All cultivars significantly showed the highest shoot regeneration on MS medium containing 6 mg/dm3 isopentenyladenine (2iP). For most rhododendrons, the highest shoot multiplication was found on a medium with 8-10 mg/dm3 2iP in combination with 1 mg/dm3 indoleacetic acid (IAA). Shoots rooted successfully in the substrate with high level of peat without growth regulators. However, the commercial preparation Racine significantly increased rooting in cv. Grandiflorum, Nova Zembla and Rebe compared with 0.03% indolebutyric acid (IBA).

Keywords: Rhododendron L.; growth regulators; shoot regeneration; multiplication; shoot rooting

Published: June 30, 2008  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Vejsadová H. Growth regulator effect on in vitro regeneration of rhododendron cultivars. Hort. Sci. (Prague). 2008;35(2):90-94. doi: 10.17221/643-HORTSCI.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. ANDERSON W.C., 1984. A revised tissue culture medium for shoot multiplication of rhododendron. Journal of the American Society for Horticultural Science, 109: 343-347. Go to original source...
    2. ECONOMOU A.S., READ P.E., 1986. Microcutting production from sequential reculturing of hardy deciduous azalea shoot tips. HortScience, 21: 137-139. Go to original source...
    3. HSIA C.H., KORBAN S.S., 1997. The influence of cytokinins and ionic strength of Anderson's medium on shoot establishment and proliferation of evergreen azalea. Euphytica, 93: 11-17. Go to original source...
    4. IAPICHINO G., CHEN T.H.H., 1995. Genotypic effects on plant regeneration from leaf segments of Rhododendron. Advances in Horticultural Science, 9: 170-172.
    5. IAPICHINO G., McCULLOCH S., CHEN T.H.H., 1992. Adventitious shoot production from leaf explants of rhododendron. Plant Cell, Tissue and Organ Culture, 30: 237-241. Go to original source...
    6. MERTENS M., WERBROUCK S., SAMYN G., SILVA H., DEBERGH P., 1996. In vitro regeneration of evergreen azalea from leaves. Plant Cell, Tissue and Organ Culture, 45: 231-236. Go to original source...
    7. MURASHIGE T., SKOOG F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15: 473-497. Go to original source...
    8. NORTON M.E., NORTON CR., 1985. In vitro propagation of Ericaceae: A comparison of the activity of the cytokinins N6-benzyladenine and N6-isopentenyladenine in shoot proliferation. Scientia Horticulturae, 27: 335-340. Go to original source...
    9. PAVINGEROVÁ D., BŘÍZA J., PRENEROVÁ E., 2000. Odvození primárních kultur z květních pupenů rododendronů. Rostlinná výroba, 46: 281-283. Go to original source...
    10. PREIL W., ENGELHARDT M., 1977. Meristem culture of azaleas (Rhododendron simsii). Acta Horticulturae, 78: 203-208. Go to original source...
    11. PROCHÁZKA S., MACHÁČKOVÁ L., KREKULE J., ©EBÁNEK J. et al., 1989. Fyziologie rostlin. Praha, Academia: 484.
    12. VEJSADOVÁ H., DERID H., 1999. Regeneration of Rhododendron L. from leaves under aseptic conditions. Biologia, 54: 27-28.
    13. VEJSADOVÁ H., PRE«OVÁ A., 2000. Cytological and histological study of morphogenesis in Rhododendron L. Advances in Horticultural Science, 14: 87-91.
    14. VEJSADOVÁ H., PRE«OVÁ A., 2003. Somatic embryogenesis in Rhododendron catawbiense Grandiflorum. Acta Horticulturae, 616: 467-470. Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content