Hort. Sci. (Prague), 2006, 33(1):7-10 | DOI: 10.17221/3733-HORTSCI

The reliability of detection and the distribution of Apple chlorotic leafspot virus in pears in the Czech Republic

J. Polák, J. Svoboda
Division of Plant Medicine, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic

The distribution of Apple chlorotic leafspot virus in pear cultivars in selected orchards of three different districts in the Czech Republic was evaluated by ELISA. The same procedure of DAS - ELISA as in apple trees, was used for the detection of ACLSV in pear trees. The detection of ACLSV in pear flower petals by ELISA was more sensitive than the ACLSV detection in leaves. ACLSV was detected in pear leaves in the reliable way only in June. The presence of ACLSV was not proved by ELISA in nine tested pear rootstocks. The different distribution of ACLSV was detected in pear cultivars Lucasova máslovka and Boscova lahvice grown in different orchards. Low occurrence of ACLSV was found in cultivars Konference, Pařížanka and Dicolor. ACLSV was not detected in cultivars Madame Verté and Bohemica.

Keywords: pear; orchards; Apple chlorotic leafspot virus; distribution; flower; cultivars and rootstocks; ELISA

Published: March 31, 2006  Show citation

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Polák J, Svoboda J. The reliability of detection and the distribution of Apple chlorotic leafspot virus in pears in the Czech Republic. Hort. Sci. (Prague). 2006;33(1):7-10. doi: 10.17221/3733-HORTSCI.
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    References

    1. CLARK M., ADAMS A.N., 1977. Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology, 34: 51-57. Go to original source... Go to PubMed...
    2. DETIENNE G., DELBOS R., DUNEZ J., 1980. Use and versatility of the immunoenzymatic ELISA procedure in the detection of different strains of an apple chlorotic leaf spot virus. Acta Phytopathologica Academiae Scientiarum Hungaricae, 15: 39-45. Go to original source...
    3. FLEGG C.L., CLARK M.F., 1979. The detection of apple chlorotic leaf spot virus by a modified procedure of enzymelinked immunosorbent assay (ELISA). Annals of Applied Biology, 91: 61-65. Go to original source...
    4. KIRKPATRICK H.C., LINDER R.C., 1964. A mechanically transmissible virus latent in apple. Phytopathology, 54: 229-232.
    5. LISTER R.M., BANCROFT J.B., SHAY J.R., 1964. Chlorotic leaf spot a mechanically transmissible virus from apple. Phytopathology, 54: 1300-1301.
    6. MARTELLI G.P., CANDRESSE T., NAMBA S., 1994. Trichovirus, a new genus of plant viruses. Archives of Virology, 134: 451-455. Go to original source... Go to PubMed...
    7. MINK G.I., SHAY J.R., 1959. Preliminary evolution of some Russian apple varieties as indicators for apple viruses. Supplement Plant Disease Reporter, 254: 13.
    8. POLÁK J., ZIEGLEROVÁ J., BOUMA J., 1997. Diagnosis and distribution of apple chlorotic leafspot virus in pome fruits in the Czech Republic. Zahradnictví - Hort. Sci. (Prague), 24: 89-94.
    9. VARVERI C., BEM F., 1995. Viruses of stone and pome fruit mother-tree plantations in Greece. Acta Horticulturae, 386: 431-438. Go to original source...

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