Hort. Sci. (Prague), 2005, 32(3):85-88 | DOI: 10.17221/3770-HORTSCI

Sporadic distribution of Plum pox virus M strain in natural sources in the Czech Republic

J. Polák, J. Pívalová

The presence and distribution of M strain of Plum pox virus (PPV-M) were investigated in natural hosts of Sharka, plums, myrobalans and blackthorns in the Czech Republic. Leaves or flowers of trees were evaluated for the presence of PPV by specific polyclonal antibodies at first. PPV infected samples were investigated for the presence of PPV-M by strain specific monoclonal antibodies. 102 PPV isolates from plum, 81 from myrobalan and 25 from blackthorn were typed. PPV-M was detected in six plum trees, six myrobalan trees and in one shrub of blackthorn. Sporadic incidence of PPV-M was proved in all investigated areas of the Czech Republic. Molecular and serological typing of different PPV strains in natural hosts, plum, apricot, and peach orchards was proposed to realize in Central Europe.

Keywords: Plum pox virus; PPV-M strain; detection; plum; myrobalan; blackthorn; monoclonal antibodies

Published: September 30, 2005  Show citation

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Polák J, Pívalová J. Sporadic distribution of Plum pox virus M strain in natural sources in the Czech Republic. Hort. Sci. (Prague). 2005;32(3):85-88. doi: 10.17221/3770-HORTSCI.
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    References

    1. BOSCIA D., ZERAMDINI H., CAMBRA M., POTERE O., GORRIS M.T., MYRTA A., DI TERLIZZI B., SAVINO V., 1997. Production and characterization of a monoclonal antibody specific to the M serotype of plum pox potyvirus. European Journal of Plant Pathology, 103: 477-480. Go to original source...
    2. CAMBRA M., ASENSIO M., GORRIS M.T., PEREZ E., CAMARASA E., GARCIA J.A., MOYA J.J., LOPEY-ABELLA D., VELA C., LANZ A., 1994. Detection of plum pox potyvirus using monoclonal antibodies to structural and non-structural proteins. EPPO Bulletin, 24: 569-577. Go to original source...
    3. CLARK M.F., ADAMS A.N., 1977. Characteristic of the microplate method of enzyme-linked immunosorbent assay for the detection of plant virus. Journal of General Virology, 34: 51-57. Go to original source... Go to PubMed...
    4. GLASA M., PALKOVICS L., KOMÍNEK P., LABONNE G., BITTNEROVÁ S., KÚDELA O., CANDRESSE T., ©UBR Z., 2004. Geographically and temporally distant natural recombinant isolates of Plum pox virus (PPV) are genetically very similar and form a unique PPV subgroup. Journal of General Virology, 85: 2671-2681. Go to original source... Go to PubMed...
    5. KERLAN C., DUNEZ J., 1979. Différentiation biologique et sérologique de souches du virus de la sharka. Annales de Phytopathologie, 7: 287-297.
    6. KOMÍNEK P., HÁK R., POLÁK J., 1998. Characterization of three Plum pox virus isolates in the Czech Republic. In: 7th International Congress of Plant Pathology, Edinburgh, 9-16 August 1998. Abstract, 2: 1.11.35.
    7. LAIMER M., MENDONCA D., ARTHOFER W., HANZER V., MYRTA A.,BOSCIA D., 2003. Occurrence of different plum pox virus strains in several stone fruit species in Austria. Options Méditerranéennes, Serie B, 45: 79-83.
    8. MALINOWSKI T., 2004. Serological characterisation of Plum pox virus (PPV) isolates found in Poland. European Meeting, 04 on Plum pox. Book of Abstracts. RogówSkierniewice, Poland: 51.
    9. NAVRÁTIL M., ©IMONOVÁ V., PAPR©TEJN F., KARE©OVÁ R., 1998. Detection and serological identification of Plum pox virus isolates in the Czech Republic. Acta Horticulturae, 472: 373-378. Go to original source...
    10. POLÁK J., 1997. On the epidemiology of plum pox virus in the Czech Republic. Ochrana rostlin, 33: 81-88. Go to original source...
    11. POLÁK J., 2002. Distribution of Plum pox virus in the Czech Republic. Plant Protection Science, 38: 98-102. Go to original source...
    12. POLÁK J., 2004. To the distribution of PPV-M strain in selected orchards of apricots and peaches in the Czech Republic. Horticultural Science (Prague), 34: 44-46. Go to original source...
    13. POLÁK J., PÍVALOVÁ J., 2001. Towards diversity of natural sources of Plum pox virus and distribution of its strain in the Czech Republic. In: Proceedings of 5th Congress European Foundation for Plant Pathology: 25-28.
    14. POLÁK J., PÍVALOVÁ J., KOMÍNEK P., MYRTA A., FORMICA L., 2003. Natural sources of Plum pox virus and typing of strains in the Czech Republic. Options Méditerranéennes, Serie B, 45: 85-87.
    15. POLÁK J., PÍVALOVÁ J., SVOBODA J., 2004. Reaction of plants of plum cv. Jojo on infection with three different strains of Plum pox virus. In: 7th Conference of EFPP. Book of Abstracts. Scotland, University of Aberdeen: 48 (141).
    16. PONCAROVÁ Z., KOMÍNEK P., 1998. Restriction fragment length polymorphism differentiation of Plum pox virus isolates. Acta Virologica, 42: 268-269. Go to PubMed...
    17. SMOLÁK J., NOVÁK J.B., 1956. Příspěvek k virologii ovocných stromů. Praha, Acta Universitatis Agriculturae: 99-118.

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