Hort. Sci. (Prague), 2005, 32(1):1-5 | DOI: 10.17221/3758-HORTSCI

Use of rooted leaves for screening of Brassica germplasm response to clubroot (Plasmodiophora brassicae) and downy mildew (Hyaloperonospora parasitica)

P. Havránek, B. Navrátilová, V. Chytilová

Rooted leaves and cotyledons of various cruciferous crops were used for the screening of Brassica germplasm response to two obligatory pathogens: clubroot (Plasmodiophora brassicae) and downy mildew (Hyalope-ronospora parasitica). The development of roots was induced after 20-hour dipping of leaf pedicels in the mixture of growth regulators: indolyl-butyric acid (10 mg/l) and nicotinic acid (5 mg/l). The detached rooted leaves and cotyledons were maintained in 250ml plastic containers with perlite under fluorescent tubes in a growth chamber. With additional foliar fertilizing they remain vital for four months, producing clubroot galls on roots when dip-inoculated with Plasmodiophora spores and sporulating mycelia of downy mildew on leaves after drop inoculation with Hyaloperonospora parasitica. The possibilities of enhancing the sensitivity of this alternative assay in combination with immunochemical methods are discussed.

Keywords: Brassica; clubroot; Plasmodiophora brassicae; downy mildew; Hyaloperonospora parasitica; rooted leaves

Published: March 31, 2005  Show citation

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Havránek P, Navrátilová B, Chytilová V. Use of rooted leaves for screening of Brassica germplasm response to clubroot (Plasmodiophora brassicae) and downy mildew (Hyaloperonospora parasitica). Hort. Sci. (Prague). 2005;32(1):1-5. doi: 10.17221/3758-HORTSCI.
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    References

    1. AGNOLA B., BOURY S., MONOT C., QUILLÉVÉRÉ A., HERVÉ Y., SILUÉ D., 2003. Evidence that a leaf-disk test allows assessment of isolate-specific resistance in Brassica oleracea crops against downy mildew (Peronospora parasitica). European Journal of Plant Pathology, 109: 471-478. Go to original source...
    2. CRUTE I.R., PHELPS K., BARNES A., BUCZACKI S.T., CRISP P., 1983. The relationship between genotypes of three Brassica species and collections of Plasmodiophora brassicae. Plant Pathology, 32: 405-420. Go to original source...
    3. KJENSEN B.D., HOCKENHULL J., MUNK L., 1999. Seedling and adult plant resistance to downy mildew (Peronospora parasitica) in cauliflower (Brassica oleracea convar. botrytis var. botrytis). Plant Pathology, 48: 604-612. Go to original source...
    4. KUDLÍKOVÁ I., SLOVÁČEK J. 2002. Preliminary report of immunochemical methods to detect resting spores of Plasmodiophora brassicae in soil. Book of Abstracts of 6th Conference of European Foundation for Plant Pathology, Czech Republic, 8-14th September 2002: 71.
    5. LEBEDA A., 1986. Pseudoperonospora cubensis. In: LEBEDA A. (ed.), Methods of testing vegetable crops for resistance to plant pathogens. Olomouc, VHJ Sempra, Research Institute of Vegetable Crops: 81-85.
    6. MORGNER M., SACRISTÁN M.D., 1995. Quantitative determination of colonization by Plasmodiophora brassicae (Wor.) in Brassica napus and Brassica oleracea. Cruciferae Newsletter, 17: 80-81.
    7. NAVRÁTILOVÁ B., BŮŽEK J., ŠIROKÝ J., HAVRÁNEK P., 1997. Construction of intergeneric somatic hybrids between Brassica oleracea and Armoracia rusticana. Biologia Plantarum, 39: 531-541. Go to original source...
    8. ROD J., 1987. Vliv pěstebních substrátů na intenzitu výskytu nádorovitosti (Plasmodiophora brassicae). Olomouc, Bulletin Výzkumný ústav zelinářský, 31: 11-17. Go to original source...
    9. ROD J., 1994. The occurrence and distribution of pathotypes of Plasmodiophora brassicae WOR. in the Czech Republic and Slovakia. Ochrana rostlin, 30: 171-182.
    10. URBAN J., LEBEDA A., 2004. Differential sensitivity of fungicides in Czech populations of Pseudoperonospora cubensis. In: LEBEDA A., PARIS H.S. (eds.), Progress in Cucurbit Genetics and Breeding Research. Proc. of Cucurbitaceae 2004, the 8 th EUCARPIA Meeting on Cucurbit Genetics and Breeding. Olomouc, Palacký University: 275-280.
    11. WILLIAMS P.H., 1985. Resource Book of Crucifer Genetic Cooperative (608-262-6496), DSTT 06-17-87 WILLPAU, Workbook of Madison College of Agricultural and Life Sciences, University of Wisconsin.

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