Hort. Sci. (Prague), 2002, 29(1):35-40 | DOI: 10.17221/4468-HORTSCI

Ploidy identification of doubled chromosome number plants in Viola × wittrockiana Gams. M 1-generation

I. Ajalin, F. Kobza, J. Doležel
1 Mendel University of Agriculture and Forestry, Brno, Faculty of Horticulture, Lednice na Moravì, Czech Republic
2 Institute of Experimental Botany, Laboratory of Molecular Cytogenetics and Cytometry, Olomouc, Czech Republic

The aim of this study was to develop a protocol for production of polyploid M1-generation plants of Viola × wittrockiana Gams. Two variants of colchicine treatment were compared for their efficiency. Early detection of novel ploidy levels was achieved by screening of stomata size, leaf index value (leaf blade length/width), and other morphological characteristics of the M1-generation. Secondary screening for novel ploidy levels was performed by flow cytometry (FCM). Hexadecaploid, aneuploid, and mixoploid plants were successfully identified by FCM.

Keywords: flow cytometry; polyploidisation; colchicine; hexadecaploids; polyploidisation efficiency; morphological characteristics; Viola × wittrockiana Gams.

Published: March 31, 2002  Show citation

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Ajalin I, Kobza F, Doležel J. Ploidy identification of doubled chromosome number plants in Viola × wittrockiana Gams. M 1-generation. Hort. Sci. (Prague). 2002;29(1):35-40. doi: 10.17221/4468-HORTSCI.
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    12. Identifikace úrovnì ploidity rostlin se zmnoženým poètem chromozomù u Viola × wittrockiana Gams. M1-generace
    13. ABSTRAKT: Ke zmnožení poètu chromozomù Viola × wittrockiana Gams. roztokem kolchicinu byly užity dvì metody, a to máèení nabobtnalých semen a aplikace na vegetaèní vrchol. Detekce polyploidù s novými úrovnìmi ploidie byla provedena primární selekcí na základì mìøení a porovnání zmìnìných morfologických znakù, jako je velikost prùduchù, délka a šíøka listové èepele (listový index), délka hypokotylu a dalších. Rozhodující selekce byla provedena prùtokovou cytometrií, která identifikovala hexadekaploidní, aneuploidní a mixoploidní rostliny. Efektivita polyploidizace i primární selekce byly zhodnoceny.
    14. Klíèová slova: prùtoková cytometrie (FCM); polyploidizace; kolchicin; hexadekaploidy; efektivita polyploidizace; morfologické znaky; Viola × wittrockiana Gams.

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