Hort. Sci. (Prague), 2002, 29(1):17-22 | DOI: 10.17221/4465-HORTSCI

Sucrase in immobilized cells of Cucumis sativus L.

S.I. Ignatova, L.M. Kušč, L. Bilisics, L. Trinh, N.N. Tiuliaeva, M.G. Sedova
1 Selection & Seed Production Agrocompany "Iljinicha, Llc", Moscow, Russia
2 Intersemia, Zolskaja, Russia
3 Institute of Chemistry, Slovak Academy of Science, Bratislava, Slovak Republic
4 State Institute of Drug Control, Hanoi, Vietnam
5 All-Russian Exhibition Centre, Closed Joint Stock Company "Rastenievodstvo", Moscow, Russia

Cell suspension cultures of Cucumis sativus L. - cucumber were permeabilized by Tween, hexadecyltri-methylammonium bromide, hexadecylpyridiniumbromide ethanol and/or immobilized by glutaraldehyde. The highest invertase activity was at pH 4.4 and temperature 53°C. The hydrolysis of the substrate was linear for 5 h reaching 60% conversion. The cells displayed high sucrase activity and convenient physico-mechanical properties.

Keywords: glutaraldehyde; sucrase; cell permeabilization; cell immobilization; Cucumis sativus L.

Published: March 31, 2002  Show citation

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Ignatova SI, Kušč LM, Bilisics L, Trinh L, Tiuliaeva NN, Sedova MG. Sucrase in immobilized cells of Cucumis sativus L. Hort. Sci. (Prague). 2002;29(1):17-22. doi: 10.17221/4465-HORTSCI.
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